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《Acta Ecologiae Animalis Domastici》 2007-04
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Identification of Major Pathogens in Bovine Mastitis by Multiplex PCR

ZHANG Shan-rui1.2,WANG Chang-fa1,GAO Yun-dong1,YANG Shao-hua1, ZHAO Hong-kun1.2,ZHONG Ji-feng1(1.Cow Research Center of Agricultural Science Academy of Shandong Province,Jinan,Shandong 250100,China;2.College of Veterinary Medicine,Shandong Agricultural University,Taian,Shandong 271018,China)  
It is important to identify the major pathogens for the antimicrobial therapy and control the rate of infection at the farm level.Three sets of specific primers were designed for the major pathogens which caused mastitis according to the published sequence of GenBank: Staphylococcus aureus,Streptococcus agalactiae,and Escherichia coli.The specificity of the assay was 100%.And the sensitivity of the assay was 1.25×103 cfu/mL.
【Fund】: 国家“十五”重大科技攻关奶业专项(2002BA518A16);; 山东省“三0”工程项目资助(2004-2006)
【CateGory Index】: S858.23
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