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《Acta Ecologiae Animalis Domastici》 2007-04
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Identification of Major Pathogens in Bovine Mastitis by Multiplex PCR

ZHANG Shan-rui1.2,WANG Chang-fa1,GAO Yun-dong1,YANG Shao-hua1, ZHAO Hong-kun1.2,ZHONG Ji-feng1(1.Cow Research Center of Agricultural Science Academy of Shandong Province,Jinan,Shandong 250100,China;2.College of Veterinary Medicine,Shandong Agricultural University,Taian,Shandong 271018,China)  
It is important to identify the major pathogens for the antimicrobial therapy and control the rate of infection at the farm level.Three sets of specific primers were designed for the major pathogens which caused mastitis according to the published sequence of GenBank: Staphylococcus aureus,Streptococcus agalactiae,and Escherichia coli.The specificity of the assay was 100%.And the sensitivity of the assay was 1.25×103 cfu/mL.
【Fund】: 国家“十五”重大科技攻关奶业专项(2002BA518A16);; 山东省“三0”工程项目资助(2004-2006)
【CateGory Index】: S858.23
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【Co-citations】
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1 CHENG Yu xin 1,WANG Bin 2,NIE Guo you 3 (1.Anhui Technical Teachers College; 2.Liuan County Livestock Center;3.Liuan Cunty Livestock Center);The conception of selecting genetic marking trait to reduce recessive mastitis of cows[J];Journal of Anhui Technical Teachers College;2003-01
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7 LI Hong-sheng, YU Jie, LI Xin-pu, LUO Jin-yin, XU Ji-ying, ZHANG Li-hua,PAN Hu, ZHANG Zhi-chang,YUAN Yong-long, YANG Yu-ying, LIU Chun-chuan, HOU Yi-zhao (Institute of Lanzhou Animal Science and Vet. Pharmaceutics,Chinese Academy of Agricultural Sciences,Lanzhou,Gansu,730050,China);Study on Relativity between Mastitis Type and Pathogenic Bacterial Infection in Dairy Cows[J];Progress In Veterinary Medicine;2004-06
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【Co-references】
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1 WANG Lin1,YANG Xue-yun1,WANG Zhi-cai1,LI Jian-jun1,CONG Fu2(1.Institute of Veterinary Medicine,Xinjiang Academy of Animal Science,Urumqi 830000,China;2.Department of Veterinary Medicine,Xinjiang Agricultural University,Urumqi 830052,China);RAPD-PCR Typing of Staphylococcus Aureus Isolated from Bovine Mastitis[J];China Animal Husbandry & Veterinary Medicine;2007-11
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