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《Acta Ecologiae Animalis Domastici》 2013-07
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Polymorphisms of SLC36A1 Gene in Yushu Horse and Debao Pony

XU Ping,HUANG Jie-ping,WANG Feng-qiao,DANG Rui-hua,LEI Chu-zhao(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China)  
The champagne coat color in horses is controlled by a single autosomal-dominant champagne gene(CH),which contains four candidate genes(SLC36A1,SLC36A2,SLC36A3,SPARC),of which a mutation in exon 2of SLC36A1gene has been linked to horse champagne coat color.To reveal the genetic diversity of SLC36A1gene in Chinese domestic horses,ten exons and partial introns sequences of 74samples including Yushu horse and Debao pony breed were sequenced using DNA pool as template.Five SNPs including intron 3(g.26699953AG,g.26699851GC,g.26699850GC),exon 4(g.26699562GA) and exon 6(g.26697018CT)were detected in two horse breeds.PCR-RFLP was used to genotype the five SNPs in 74samples of Yushu horses and Debao pony breed,showing CC and CT genotypes in exon 6,GG and GA genotypes in exon 4,GG and GC genotypes in exon 3of g.26699850GC.The remaining 2 SNPs(g.26699953AG,g.26699851GC)showed AA、AG、GG and GG、GC、CC genotypes respectively using sequencing genotyping.The 5SNPs indicated that the wild genotypes are the dominant genotypes.Nine haplotypes(H1-H9)were determined based on the genotype and H5was the dominant haplotype.The haplotype diversity(H=0.4190)of Debao pony was richer than that of Yushu horse(0.2228),indicating the genetic diversity of Debao pony is more abundant.The average haplotype diversity is 0.3160 showing the two horse breeds have low genetic diversity in champagne coat color.
【Fund】: 国家自然科学基金项目(31072001)
【CateGory Index】: S821
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 MAO Chun-chun,SUN Wei,LIU Dong-hua,XU Ping,LEI Chu-zhao(College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi 712100,China);KIT Gene Polymorphisms of Debao Pony and Hazakh Horse[J];Acta Ecologiae Animalis Domastici;2012-03
【Secondary Citations】
Chinese Journal Full-text Database 1 Hits
1 BAI Dong-Yi1,YANG Li-Hua2,Unerhu1,ZHAO Yi-Ping1,ZHAO Qi-Nan1,Hasigaowa3,Dugarjaviin Manglai1 1.College of Animal Science,Inner Mongolia Agricultural University,Hohhot 010018,China;2.College of Life Science,Inner Mongolia Agricultural University,Hohhot 010018,China;3.Bureau of Agricultural and Livestock,West Ujimqin of Xilin Gol League,Inner Mongolia,West Ujimqin 026200,China;Effects of Kit gene on coat depigmentation in white horses[J];Hereditas;2011-11
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