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《Scientia Sinica(Vitae)》 2014-04
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Application of Next-Generation Sequencing Technology for Plant Virus Identification

QIAN YaJuan;XU Yi;ZHOU Qi;ZHOU XuePing;State Key Laboratory of Rice Biology,Institute of Biotechnology,Zhejiang University;State Key Laboratory of Rice Biology, Institute of Biotechnology,Zhejiang University;Inspection and Quarantine Technology Center,Beijing Entry-Exit Inspection and Quarantine Bureau;  
Next-generation sequencing technology(NGS) has been used as a powerful tool for studies on plant pathogens, especially for plant virus identification. NGS is sequence-independent and culture-independent approach, which can be used for detection of RNA viruses, DNA viruses and viroid simultaneously in a plant sample which contains very low titers. Diagnostic for plant viruses is easy by using this revolutionary technology. In the past few years, NGS has been successfully used for survey and identification of viruses in numerous plant species. In this review, we discuss the application and future perspective of NGS in plant virus identification.
【Fund】: 质检公益性行业科研专项(批准号:201310068)资助
【CateGory Index】: S432.41
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