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《Basic Medical Sciences and Clinics》 2005-02
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Inhibitory effect of CCK-8 on the expressions of TLR4 and IL-1β in LPS-induced pulmonary interstitial macrophages of rats

NI Zhi-yu, LI Shu-jin, CONG Bin, YAO Yu-xia, WANG Chun-yan(The School of Basic Medical Sciences,Hebei Medical University,Shijiazhuang 050017,China)  
In order to study the effect of cholecystokinin octapeptide(CCK-8)of inhibiting the expression of TLR4 and IL-1β in rat pulmonary interstitial macrophages (PIMs) induced by lipopolysaccharide. Methods PIMs was isolated and cultured in the presence or absence of LPS, CCK-8, and vehicle. The expression of TLR4 mRNA and IL-1β mRNA was assayed by Northern blot and RT-PCR. IL-1β in the supernatant of culture media was detected by ELISA. Results TLR4 mRNA and IL-1β mRNA in rat PIMs were up-regulated by LPS(1mg/L). IL-1β production were increased by LPS incubation in a time-dependent manner. CCK-8, at concentrations of 10 -7 and 10 -6 mol/L, significantly inhibited the production of IL-1β and the expression of TLR4 mRNA and IL-1β mRNA. Conclusion The results indicated that CCK-8 negatively modulated the expression of TLR4 and IL-1β in rat PIMs induced by LPS. This may be one of the mechanisms for CCK-8 to alleviate inflammation in lung tissue during endotoxemia.
【Fund】: 河北省自然科学基金 (30 0 32 2)
【CateGory Index】: R363.2
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【Co-citations】
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