Expression and regulation of HES1 in sperms with low motility from asthenospermia patients

LI Yan1,WEN Liu2,CHEN De-yu2,3(1.Analytical Cytology Laboratory;2.Research Center for Reproductive Medicine,Shantou University Medical College,Shantou 515041;3.Fuyang Teachers College,Fuyang 236041,China)  

Objective To analyze the expression and regulation of HES1 in sperms with low motility.MethodsThirty semen samples from asthenospermia patients and 20 semen samples from healthy and fertile adults were collected,total RNAs were extracted to produce cDNAs probes.Hybridization with Phalanx OneArrayTM containing 30 968 probes was carried out after the labeled cDNAs were purified by PCR product purification kit.Realtime RT-PCR was used to analyze the expression of hsa-miR-487a and hsa-miR-193b;the expression of the target genes of hsa-miR-487a and hsa-miR-193b were searched from gene-expression profiles in asthenospermia patients' sperms.Results The expression level of HES1 in low motility sperms was up-regulated.The expression level of hsa-miR-193b in low motility sperms was 2.19 times higher than that in high motility sperms,hsa-miR-487a was 0.43% of that in high motility sperms.Conclusion The expression level of HES1 in low motility sperms was up-regulated.Hsa-miR-487a and hsa-miR-193b may affect the expression of HES1 and so regulate sperm motility.

【Key Words】： asthenospermia sperm HES1 hsa-miR-487a hsa-miR-193b
【Fund】： 广东省自然科学基金(81515031020000027);; 广东省科技计划项目(2008B03031242)
【CateGory Index】： R698.2
【DOI】： CNKI:SUN:JCYL.0.2010-05-014

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