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《Basic & Clinical Medicine》 2016-12
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siRNA silencing EGFR expression alleviates brain injury stems from intracerebral hemorrhage of rats

QIAN Hong;LIU Li-jing;XIE Ming;WU Heng;LIU Shuang-xi;WU Bin;XIANG Hai;School of Clinical Medicine,Hunan University of Medicine;Dept.of Neurology,the First Affiliated Hospital,University of South China;Dept.of Neurology,Huaihua First People's Hospital;  
Objective To explore the effects of siRNA for epidermal growth factor receptor( EGFR) on rat brain injury after intracerebral hemorrhage and its related mechanisms. Methods The intracerebral hemorrhage model was established by injecting of Ⅶ collagenase into the caudate nucleus in rats. Rats were then treated with injection of 10 μL empty viral vector or EGFR siRNA lentivirus expression vector into lateral ventricles. The neurological function score was evaluated,and HE staining was used to observe the pathological changes of cerebral tissues.Water content in brain was examined by the dried and wet weight method. EGFR expression was measured using immunohistostaining. Glial fibrillary acidic protein( GFAP) mRNA level was analyzed through fluorescence realtime quantitative PCR. Western blot was used to detect EGFR,GFAP,signal transducers and activators of transcription 3( STAT3) and phosphorylated STAT3( p-STAT3). Results With the extension of intracerebral hemorrhage time,ERGF expression in cerebral tissues around hemotomas was gradually upregulated,and reached thepeak on day 7( P 0. 01). In comparison with model group,EGFR siRNA relieved pathological impairment of cerebral tissues,reduced neurological function score and brain water content,and decreased the expression levels of EGFR,GFAP and p-STAT3 in cerebral tissues around hemotomas( P 0. 01). Conclusions Gene silence of EGFR inhibits the activation of astrocytes through blockade of STAT3 phosphorylation,thus leading to reduction of brain injury after intracerebral hemorrhage.
【Fund】: 湖南省教育厅资助科研项目(14C0906)
【CateGory Index】: R743.34
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【Citations】
Chinese Journal Full-text Database 4 Hits
1 QIAN Hong;LIU Li-jing;LI Yan-chun;XIE Ming;WU Heng;LIU Shuang-xi;WU Bin;School of Clinical Medicine,Hunan University of Medicine;Dept of Neurology,the First Affiliated Hospital,Hunan University of Medicine;Dept of Neurology,the First Affiliated Hospital,University of South China;Dept of Neurology,Huaihua First People's Hospital;;EGFR siRNA inhibits activation of astrocytes derived from rats through blockade of STAT3 phosphorylation[J];中国药理学通报;2016-02
2 XIAO Lin;LIU Zhen;WU Heng;Department of Neurology,Affiliated No1 Hosipital,Nanhua University;;EGFR-JAK1/STAT3 Expression in activated astrocytes after intracerebral hemorrhage and inhibition effect of genistein[J];中风与神经疾病杂志;2015-10
3 Kim Mai Lee;Andrew G Mac Lean;Department of Microbiology and Immunology,Tulane University School of Medicine;;New advances on glial activation in health and disease[J];World Journal of Virology;2015-02
4 GUO Zhi-hui,WU Heng.(Department of Neurology,the First Affiliated Hospital of Nanhua University,Hengyang 421001,China);The relationship of the activity of astrocytes and the expression of EGFR[J];中风与神经疾病杂志;2010-08
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 QIAN Hong;LIU Li-jing;XIE Ming;WU Heng;LIU Shuang-xi;WU Bin;XIANG Hai;School of Clinical Medicine,Hunan University of Medicine;Dept.of Neurology,the First Affiliated Hospital,University of South China;Dept.of Neurology,Huaihua First People's Hospital;;siRNA silencing EGFR expression alleviates brain injury stems from intracerebral hemorrhage of rats[J];基础医学与临床;2016-12
2 DAI Jin-quan;CHEN Yan-hui;Department of Pediatrics,Fuzhou Children's Hospital of Fujian Province;Department of Pediatrics,Fujian Medical University Union Hospital;;The Study of Specific Biological Markers on Brain Injury of Epilepsy[J];医学综述;2016-21
3 GUO Min-fang;ZHAGN Hui-yu;LIU Hua;Department of histology and embryology, Medical School, Shanxi Datong University;;In Astrocyte Stimulated with Lipopolysaccharide[J];中外医疗;2016-17
4 DONG Li-sha;LI Xue-hui;LIU Wen-li;ZHAO Peng;YAO Bi-yun;ZHOU Zong-can;School of Public Health,Peking University;;The activation of primary astrocytes induced by manganese chloride[J];毒理学杂志;2016-01
5 LUO Xiao-jin;LIN Mao;WANG Chun-mei;Department of Physiology,Zhuhai Campus of Zunyi Medical University;;Role and research progress of inflammation in the mechanism of Alzheimer′s disease[J];热带医学杂志;2016-01
6 QIAN Hong;LIU Li-jing;LI Yan-chun;XIE Ming;WU Heng;LIU Shuang-xi;WU Bin;School of Clinical Medicine,Hunan University of Medicine;Dept of Neurology,the First Affiliated Hospital,Hunan University of Medicine;Dept of Neurology,the First Affiliated Hospital,University of South China;Dept of Neurology,Huaihua First People's Hospital;;EGFR siRNA inhibits activation of astrocytes derived from rats through blockade of STAT3 phosphorylation[J];中国药理学通报;2016-02
7 DENG Zhi-jing;GUO Zhe;PANG Yi-min;Guangxi Medical University Pharmaceutical School;;Activating rules of astrocytes and microglias in mice after cerebral ischemia[J];中华老年心脑血管病杂志;2016-01
8 XIAO Lin;LIU Zhen;WU Heng;Department of Neurology,Affiliated No1 Hosipital,Nanhua University;;EGFR-JAK1/STAT3 Expression in activated astrocytes after intracerebral hemorrhage and inhibition effect of genistein[J];中风与神经疾病杂志;2015-10
9 YANG Jing-hua;GUO Wei-xu;L Dan;WU Sheng-wen;LU Xiao-bo;JIN Cui-hong;CAI Yuan;Department of Toxicology, College of Public Health, China Medical University;;Effects of lanthanum chloride on endoplasmic reticulum stress and apoptosis in astrocytes of rats[J];环境与健康杂志;2015-08
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【Secondary Citations】
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2 Kim Mai Lee;Andrew G Mac Lean;Department of Microbiology and Immunology,Tulane University School of Medicine;;New advances on glial activation in health and disease[J];World Journal of Virology;2015-02
3 WU Jie;WU Hong-hai;YU Yang;QIN Ya-bin;HAN Xiao-lei;HOU Yan-ning;Dept of Pharmacy,Bethune International Peace Hospital of PLA;Dept of Pharmacology,Hebei Medical University;;Progesterone protects neurons against impairment induced by Aβ activated astrocytes[J];中国药理学通报;2014-11
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7 Yan WU1,2, Ren-Gang LIU1, Jie-Ping ZHOU1 1Department of Anatomy, 2Department of Neurology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China;Effect of ciliary neurotrophic factor on activation of astrocytes in vitro[J];Neuroscience Bulletin;2006-06
8 YU Xiao-tang,ZHU Shi-neng,ZHU Teng-fang,CHEN Jie-qing,LU Shi-lun(Department of Pathology,Shanghai Medical College,Fudan University.Shanghai,200032,China);Role of EGFR-STAT3 signal pathway in carcinogenesis of experimental hepatoma in rats[J];中国癌症杂志;2006-02
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