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《Chinese Journal of Polar Research》 2012-02
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OPTIMIZATION OF GROWING CONDITIONS AND A METHOD FOR THE EXTRACTION OF CRUDE CAROTENOIDS PRODUCED BY RHODOCOCCUS SP.(STRAIN B7740) ISOLATED FROM THE ARCTIC SEA

Zhang Jiarong1,Yang Jifang2,3,Chen Fusheng1 1College of Food Science and Technology,Huazhong Agricultural University,Wuhan 430070,China;2Key Laboratory of Microbiology and Environmental Engineering,Ningbo 315100,China;3College of Biological and Environmental Sciences,Zhejiang Wanli University,Ningbo 315100,China)  
Rhodococcus sp.(strain B7740) isolated from the Arctic Ocean can accumulate large numbers of yellow pigments,but the biomass of these bacteria is very low.In this study,an optimal growing medium was developed using single factor and orthogonal experiments on carbon and nitrogen sources,temperature,initial pH and inoculum concentration.On this same basis,extraction conditions were optimized for the type of extraction solvent,pH,extraction time and solid to liquid ratio.Five grams of yeast extract,5 g of glucose,3 g of lactose and 7 g of beef extract were added to 1 L of old sea water.With an initial pH of 7,a temperature of 25℃ and an inoculum concentration of 1.0%,the sea water was cultured for 7 days on a shaker at a speed of 160 r/min and the biomass of B7740 increased from 6.1 mg/mL to 23.08 mg/mL,an increase of 278.4%.Dry cells were used for the experiment and anhydrous ethanol at pH7 was used as the extracting reagent with a ratio of sample to solution of 1∶30,temperature was set at 75℃ and the extraction time was 1 h,repeated twice.Using this method,the output of the pigment reached 1 160.625 μg/g dry cells.Analysis showed that the pigment was carotenoid.The results of this experiment provide the foundation for the further study of extraction methods for carotenoids produced by the polar microbe,Rhodococcus sp.(strain B7740).
【Fund】: 国家科技部国际合作重点项目(2007DFA21300);; 中德政府海洋双边合作项目(BMBFCHN00/019);; 宁波市科技局项目(2004C100056 2007C11002)资助
【CateGory Index】: Q93
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