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《Pharmaceutical Journal of Chinese People's Liberation Army》 2006-04
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Anti-respiratory Syncytial Virus Effect of the Extraction of Chrysanthemum Indicum in Vitro

ZHANG Zhen-Ya,FANG Xue-Ping,DIAO Zhi-Hua,ZENG Rui-Hong,MEI Xing-Guo (Institute of Pharmacology and Toxicology,Academy of Military Medical Sciences,Beijing 100850 China)  
Aim To evaluate the effect of anti-respiratory syncytial virus of the extraction of Chrysanthemum indicum in vitro.Methods The cytotoxicity and antiviral activity of the extraction of Chrysanthemum indicum were tested by the plaque reduction method and 3-(4,5-dimethylthiazole-2-yl) 2,5-diphenyl tetrazolium bromide) MTT assay,with ribavirin treat as the positive control drug.Results The median effective concentration (50% effective concentration,EC_ 50 ) and the median cytotoxicity concentration (50% cytotoxicity concentration,CC_ 50 )of the extraction of Chrysanthemum indicum were (60.9±2.41)μg·ml~ -1 and (2.03±0.08)mg·ml~ -1 ,respectively.The selectivity index [SI] (CC_ 50 for HEp-2:EC_ 50 ) of it for RSV was 34.SI4 is considered significant.Moreover,the extraction of Chrysanthemum indicum gave time-course experiments in inhibiting RSV.In this assay testing its effect of on virual growth,it showed obvious effect of inhibition on RSV when the herb was added at 2,4,6h and 8h after infection (P0.01).By an inhibition assay for virus absorption and penetration during the contact between the virus and cells,the extraction of Chrysanthemum indicum inhibited RSV absorption on the cells (P0.01) and penetration into the cell (P0.01).The extraction of Chrysanthemum indicum also displayed direct inactivation on RSV.Conclusion The extraction of Chrysanthemum indicum showed potent inhibition to RSV in vitro.
【CateGory Index】: R285
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【Citations】
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1 FANG Xue-Ping, ZHANG Zhen-Ya, ZENG Rui-Hong, DIAO Zhi-Hua, MEI Xing -Guo~* (Institute of Pharmacology and Toxicology, Academy of Military Medical Sciences,Beijing 100850,China);Improvements in methods for screening potential antiviral agents agains t respiratory syncytial virus[J];Bulletin of The Academy of Military(Medical Sciences);2005-04
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