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《Pharmaceutical Journal of Chinese People's Liberation Army》 2006-06
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Mitochondria-dependent Mechanisms Involved in HepG2 cell Apoptosis Induced by Uncarinic Acid E

ZHAO Ming-Hong~ ①② ,GUO Tao,WANG Min-Wei~②,ZHAO Qing-Chun (~①The General Hospital of Shenyang Military Region, Shenyang 110016,Liaoning China) (~②The school of pharmacy, Shenyang Pharmaceutical University, Shenyang 110016,Liaoning China)  
Aim To study the mechanisms of uncarinic acid E-induced HepG2 cell apoptosis. Methods Cytotoxicity assay by MTT, observasion of morphology, LDH release and Western blot analysis were carried out. Results Uncarinic acid E inhibited HepG2 cell growth in a dose- and time-dependent manner, and did not show inhibitory action on marrow in mice in vitro. Apoptotic bodies were observed in Hoechst33258 fluorescein stain. LDH assay proved that apoptosis and necrosis undertook simultaneously in death of HepG2 cell treated with uncarinic acid E. Up-regulation of Bax and down-regulation of Bcl-2 and Bcl-xL expression contributed to uncarinic acid E induced HepG2 cell apoptosis, and led to release of cytochrome c. Conclusion Mitochondria-dependent signal transduction pathways are involved in uncarinic acid E-induced HepG2 cell apoptosis.
【CateGory Index】: R735.7
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【Co-citations】
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1 XU Du-juan,CHEN Min-zhu (The Department of Pharmacy,the First Affiliated Hospital of Anhui Medical University,Anhui Hefei 230022, China);Protection of Total Astragalus Extract Against Chemotherapy-induced Leukopenia and Immunosuppression in Tumor Bearing Mice[J];Journal of Anhui Traditional Chinese Medical College;2006-03
2 ZHU Guo-fu,YANG Yan-long,HOU Ai-jun,ZHANG Hui-qing,LI Qiu-shuang,ZHANG Cong-cong(1.Department of Chinese Materia Madica,Shanghai University of Traditional Chinese Medicine,Shanghai 201203,China);Inhibitory effects of ACR-2 and ACR-3 on SMMC-7721 and SGC-7901 cell lines and enzyme activity of Caspase-3[J];Journal of Traditional Chinese Medicine University of Hunan;2007-05
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1 WANG Yu-jie,ZHANG Yi~*,DU Juan,YANG Li-juan,ZHANG Qi,SUN Qian-qian,FAN Yuan (College of Traditional Chinese Pharmacy,Chengdu University of TCM,Chengdu 611731,China);Study on the anti-inflammatory active constituents of Prinsepia utilis[J];West China Journal of Pharmaceutical Sciences;2006-02
【Secondary Citations】
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1 (Affiliated Tumour Research Institute of Guangxi Medical University Nanning,Guangxi,PR,China(530027))Quan Dafang,Li Zuoqing;CLINICAL OBSERVATION ON THE EFFECT OF TREATMENT OF STRENGTHENING THE SPLEEN AND TONIFYING THB KIDNEYS ON LMPAIRMENT OF RENAL FUNCTION CAUSED BY CHEMOTHERAPY OF DDP[J];GUANGXI JOURNAL OF TRADITIONAL CHINESE MEDICINE;1994-05
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