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《Pharmaceutical Journal of Chinese People's Liberation Army》 2010-02
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Quantitative Determination of Kakonein in Compound Geqing Ganmao Capsule by High Performance Liquid Chromatography

YANG Bi-xian①,YUN Xue-lin②①Department of Biology and Environment,Guiyang University,Guiyang 550005,China;②Prescription and TCM Office,Department of Pharmacy,Guiyang College of Traditional Chinese Medicine,Guiyang 550002,China  
Objective To determine the content of Kakonein in Compound Geqing Ganmao Capsule. Methods The high performance liquid chromatography analysis was carried out on Kromasil C18 column (250 mm×4.6 mm,5 μm),The mixture of methanol-water (25∶75)was used as the mobile phase. The flow rate was 1.0 ml/min.The detection wavelength was at 250 nm.Results The linear range of Kakonein was 30-300 μg while the average recovery was 97.64% and the RSD was 1.33%.Conclusion The method is accurate,simple and repeatable, which can be applied to quantitative determination of Kakonein in Compound Geqing Ganmao Capsule.
【CateGory Index】: R286.0
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