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《MEDICAL JOURNAL OF CHINESE PEOPLE'S LIBERATION ARMY》 2000-02
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CLONING AND EXPRESSION OF ENDOSTATIN GENE AND IDENTICATION OF ITS ANTI GLIOMAACTIVITY IN MOUSE

Qu Yan, Zhang Xiang, Wu Jingwen et al. Xijing Hospital, The Fourth Military Medical University, Xi′an 710033  
The cDNA encoding mouse endostatin was amplified by RT PCRand sequenced after cloned into vector pUC19. Then the DNA fragment was cloned into the E. coli. expression vector pDH. After expression, the purified and refoldedprotein was injected directly into the glioma of rat to indentify its anti glioma activity. The result indicated that the sequence of the amplified DNA fragment was consisted with the known gene.After induction, a new band corresponding to the 20kDa protein was observed in the bacteria carrying pDH Endo on SDS PAGE gel.The recombinant protein is highly suppressive to the glioma in rat.
【Fund】: 国家自然科学基金!资助课题(编号39970752)
【CateGory Index】: R730.5
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