INFLUENCE OF EMP IRRADIATION ON LDH, AST, CHE, K+ AND Na+ EN SUPERNATANT OF CULTURED RAT PI- TUITARY CELLS
Cao Xiaozhe, Zhao Meilan, Wang Dewen. Institute of Radiation Medicine, Academy of Military Medical Sciences, Beijing 100850
By observing the changes in LDH、AST、CHE、K、Na+ contents in supernatant of cultured rat pituitary cells after electromagnetic pulse (EMP) irradiation the injury mechanism to pituitary cells was explored. Pituitary cells of Wistar rat was cultured in 6-hole plates. The cells in five plates were irradiated with high field strength EMP ( electromagnetic pulse) for 5 times within 2 minutes. The electromagnetic pulse simulator provided a high electric field intensity of 60 KV/m, with rise time 20-nsec and pulse wide 30/μS. Contents of LDH, AST, CHE, K+ and Na+ in the supernatant were measured using reagent boxes (Beijing Zhongsheng High-tech Bioengineering Company) at Oh, 1h, 6h, 12h and 24h following irradiation, respeetively. All data were analyzed by statistical software Spss 8. 0. The results showed that LDH and CHE contents in the supernatant increased significantly at Oh after irradiation with EMP (P0. 05), contents of LDH, AST, CHE, K+and Na+ increased significantly at 1h and 6h after irradiation (P0. 01) , and contents of LDH, AST and K+ increased significantly at 12h after irradiation (P0. 05). However, contents of all of them were not statistically different from those of the controls at 24h after irradiation (P 0. 05). Our conclusion is EMP could injure membranes of pituitary cells.