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《Medical Journal of Chinese People's Liberation Army》 2003-02
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EFFECTS OF LIPOPOLYSACCHARIDE ON β-ADRENOCEPTOR AND β-ADRENOCEPTOR KINASES IN RAT PULMONARY MICROVASCULAR ENDOTHELIAL CELLS

Xiao Zhenliang, Qian Guisheng, Sun Gengyun et al . Insititute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China  
In order to probe the effects of lipopolysaccharide(LPS) on β adrenoceptor (β AR) and β AR kinases in rat pulmonary microvascular endothelial cells (RPMVEC), radioactive ligand binding assay was used to measure the distribution density of β AR before and after LPS acting in it; Western blot was used to observe the expression changes in β AR kinases, including GRK2 and GRK3. The results showed that the maximum binding capacity (Bmax) of RPMVEC β AR was 5 583±0 306 fmol/10 5 cells. The Bmax of β AR in LPS group significantly decreased compared with normal control group ( P 0 05) and no significant difference was found between normal control group and LPS+anisodamine group. The expression of GRK2 in LPS group and LPS+anisodamine group increased obviously compared with normal control group and no GRK3 expression was found in RPMVEC. The results suggest that the increased expression of GRK2 but not GRK3 induced by LPS in RPMVEC may promote the internalization of β AR, which further leads to the down regulation of β AR. Anisodamine may inhibit the down regulation of β AR through other mechanism instead of inhibiting the expression of GRK2.
【Fund】: 国家自然科学基金资助课题 (编号 30 0 70 334 ;39730 2 1 0 )
【CateGory Index】: R363
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