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《Medical Journal of Chinese People's Liberation Army》 2003-11
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THE EFFECT OF UBIQUITIN-PROTEASOME PATHWAY ON NUCLEAR FACTOR KAPPA B ACTIVITY IN LIVER OF BURN RATS WITH SEPSIS

YIN Hui nan, CHAI Jia ke, WU Yan qiu et al . Department of Burns and Plastic Surgery, Burns Institute, 304 Hospital of PLA, Beijing 100037, China  
Objevtive To study the effect of ubiquitin proteasome pathway inhibition on NF κB activity and IκBαexpression as well as TNF α secretion in the liver in burned rats with sepsis. Methods Rats were subjected to 30% full thickness scald injury, followed by intraperitoneal injection of lipopolysaccharide (LPS), was used for this experiment to mimic early sepsis after burn. Sixty Wistar rats were randomly divided into normal control group, burn sepsis group, burn sepsis with proteasome inhibitor N Acetyl leucinyl leucinyl norleucinal (ALLN) treatment group, and burn sepsis with NF κB inhibitor Pyrrolidine Dithiocarbamate (PDTC) treatment group with 6 rats in each group. The following parameters were measured with the method indicated in parentheses: NF κB activity (electrophoretic mobility shift assay, EMSA), IκB α expression (Western blotting), and TNF α (Enzyme linked immunoadsordent assay, ELISA). Results These showed that NF κB activity was markedly activated and reached its peak 1h after challenge ( P 0 05). Expression of IκB α was decreased at first and then increased to wards normal level. Degradation of IκBα was inhibited by the proteasome inhibitor ALLN. NF κB activity and TNF α secretion were found to be decreased after the administration of proteasome inhibitor ALLN or NF κB inhibitor PDTC. Conclusion The results suggested early treatment with inhibitor of ubiquitin proteasome pathway might decrease the NF κB activity of the liver in burned rats with sepsis through inhibiting degradation of IκBα
【Fund】: 国家自然科学基金(编号30271339、39970716);; 全军医学科研“十五”计划(编号01Z095)资助课题
【CateGory Index】: R644
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