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《Medical Journal of Chinese People's Liberation Army》 2008-12
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Supression effects on growth of human breast carcinoma cell MCF-7 by 15d-PGJ2,an activator of γ-receptor activated by peroxisome proliferator,and transfection in vitro of PTEN plasmid

Xu Xiao,Ma Binlin,Bai Jingping,et al.Department of Breast,Head and Neck Surgery,Department of Breast and Neck Oncology Surgery,Affiliated Tumour Hospital,Xinjiang Medical University,Urumqi 830011,China  
Objective To explore the inhibitory effects of 15d-PGJ2 and transfection of PTEN plasmid on the human breast carcinoma cell line MCF-7 in vitro.Methods MCF-7 cells were seeded on a 96-well plate in the concentration of 5.0×106/L.According to the factorial design the cells were assigned into 4 groups.1) Transfection group: MCF-7 cells were transfected with 1μg of pcDNA3.0-PTEN plasmid;2) Combination group: MCF-7 cells were transfected with pcDNA3.0-PTEN plasmid and treated with 40μmol/L 15d-PGJ2,an activator of γ-receptor activated by peroxisome proliferator(PPAR-γ);3) Interference group: MCF-7 cells were treated with 40μmol/L 15d-PGJ2;and 4) Normal group: normal cells.MTT assay was used to assess the inhibitory effects of 15d-PGJ2 and transfection of pcDNA3.0-PTEN on the growth of MCF-7 cells.Flow cytometry was employed to determine the cell cycle and apoptosis.Results It was revealed by the absorbent value(A) determined with MTT that,compared with the normal group,the growth of MCF-7 cells were efficiently suppressed in transfection group and interference group,and the suppression was even more evident in combination group at the time points of 48,72 and 96h(P0.05).In view of the inhibitory rate calculated with A value,the cell growth in normal group was not suppressed at all the time points.At 24h time point,the inhibitory rates in transfection group,combination group and interference group were 2%,0% and 0%;at 48h time points,it was 28%,39% and 26%,respectively,and at 72h time points,it was 74%,84% and 70%,respectively,and at 96h time points,it was 85%,89% and 80%,respectively.It was shown by the cell cycle detection that,compared with normal group,the growth of more cells was blocked in transfection group,and less cells in interference group in G0 and G1 stages(P0.05).In the combination group,the blocking effect on the cell growth at G0 and G1 stage was less marked compared with that in transfection group(P0.05),but was stronger than that in intereference group(P0.05).Compared with the normal group,apoptosis of MCF-7 was not induced in transfection group,combination group and interference group(P0.05).Conclusion The growth of MCF-7 cells cultured in vitro may be effectively inhibited by the combination of PTEN transfection and targeted drug 15d-PGJ2,but no apoptosis was induced.
【Fund】: 新疆少数民族科技人才特殊培养计划科研项目(200823114);; 新疆维吾尔自治区教育厅高校青年教师启动基金项目(XJEDU2006525)
【CateGory Index】: R737.9
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