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《Medical Journal of Chinese People's Liberation Army》 2009-05
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Effects of ulinastatin on NF-κB expression in lung tissue of rats with acute lung injury

JIANG Jing-jing,SHI Xue-yin,YUAN Hong-bin,ZHU Qiu-feng,WANG Cheng-cai,SUN Hai-jing.Department of Anesthesiology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China  
Objective To study the mechanism of protective effect of ulinastatin(UTI) on lung with LPS-induced acute lung injury(ALI) in rats.Methods Thirty-six male SD rats were randomly divided into three groups: normal control group(NC),lipopolysaccharide injury group(LPS) and UTI treatment group(UTI).ALI model of rats was reproduced with LPS.Rats in LPS group and UTI group received injection of LPS 5mg/kg via femoral vein,and then the rats in UTI group received injection of UTI 50 000U/kg.Rats in NC group received an injection of 2ml 0.9% NaCl.PaO2 was detected for all the three groups 2h and 4h after injection.Half of rats in each group were then sacrificed by exsanguination,and lungs were harvested for the assessment of lung wet/dry weight ratio(W/D),MPO activity and MDA level.The pathological changes in lung tissues were examined with HE-stained lung sections.TNF-α protein was measured by ELISA,and the expression of NF-κB was examined by immunohistochemistry.Results The W/D ratio of lung tissue was significantly increased in the rats of LPS and UTI group compared with that of NC group(P0.05),while no significant difference existed between LPS and UTI groups(P0.05).PaO2 decreased significantly in LPS group at 2h and 4h time points after injection compared with that in NC group(P0.01).PaO2 was higher in UTI group at each time point compared with that in LPS group(P0.05),while lower than that in NC group(P0.05).No significant difference in PaCO2 was found between UTI and LPS groups(P0.05).Marked histological damage was found in the lungs of rats of LPS group characterized by destruction of lobules,neutrophil infiltration and hemorrhage in alveolar spaces.Similar changes were also found but in a mild degree in the rats of UTI group.MPO activity and MDA level were significantly higher in LPS group than that in NC and UTI group(P0.05).The expressions of NF-κB and TNF-α were down regulated in UTI group compared with those in LPS group at the same time points(P0.05).Conclusion In LPS-induced acute lung injury,early administration of UTI could inhibit the activation of neutrophils and NF-κB,down-regulate the production of TNF-α,and play a protective effect on lung.
【CateGory Index】: R563.8
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【References】
Chinese Journal Full-text Database 3 Hits
1 PENG Xia,MA Jianfei,LI Chengcheng,et alDepartment of Nephrology,The First Affiliated Hospital China Medical University,Shenyang (110001);Effect of Ulinastatin on Lipopolysaccharide-induced Interleukin 15,Interleukin 6 Expression in Rat Peritoneal Mesothelial Cells[J];Chinese Journal of Integrated Traditional and Western Nephrology;2010-10
2 LI Ya-qing,XU Wu-lin,YAN Jian-ping,XIA Ying-jie,WANG Hong.Department of Respiratory Medicine,Zhejiang Provincial People's Hospital,Hangzhou 310014,China;Effect of Toll-like receptor-4 and NF-κB on expression of matrix metalloprotease 9 induced by lipopolysaccharide in human airway epithelial cells[J];Medical Journal of Chinese People's Liberation Army;2011-10
3 CHEN Xiao-bing,XU Tie,LI Xiao-min,et al.Center of Emergency,First People's Hospital of Lianyungang,Affiliated Hospital Xuzhou Medical College,Lianyungang 222001,China;Explore the Effection and Machanism of Different Doses of Ulinastatin in Septic[J];Journal of Clinical Rational Drug Use;2011-29
【Citations】
Chinese Journal Full-text Database 5 Hits
1 LI Qi, QIAN Gui sheng, ZHANG Qing, WANG Chang zheng (Institute of Respiratory Diseases, Xingqiao Hospital, Third Military Medical University, Chongqing 400037, China);Effects of different doses of lipopolysaccharide on acute lung injury in rats[J];Acta Academiae Medicinae Militaris Tertiae;2004-10
2 QIAN Gui-sheng. Institute of Respiatory Disease of PLA,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China;Recent progress in research on the diagnosis and treatment of acute lung injury and acute respiratory distress syndrome[J];Medical Journal of Chinese People's Liberation Army;2009-04
3 Guo Zhenghui,Hong Xin,Mao Baoling,et al.Department of Instisute of Respiratory Disease,Xingqiao Hospital,Third Military Medical University,Chongqing 400037,China [;Effects of nuclear factor-κB activation on acute lung injury[J];Journal of Emergency Medicine;2003-09
4 SHEN Jin-mei, HE Xiao-jing, et al. Department of Anesthesiology, Second Hospital of Xiangya , Central South University, Changsha 410011, China;Effects of ulinastatin on cytoldnes and respiratory index during open heart surgery with CPB[J];Chinese Journal of Anesthesiology;2004-08
5 SHAO Yiming, ZHANG Liangqing, DENG Liehua, YAO Huaguo. Department of Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong, China;Clinical study on effects of ulinastatin on patients with systemic inflammatory response syndrome[J];Chinese Critical Care Medicine;2005-04
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 ZHANG Zi-jun,CUI Ming,JIANG Yong,ZUO YAN-qing (Tongling People's Hospital,Tongling,Anhui 244009,China);Therapeutic value of ulinastatin in acute severe traumatic craniocerebral injury[J];Anhui Medical and Pharmaceutical Journal;2009-10
2 WANG Chun-hua,HU De-lin,YU You-xin,FANG Lin-sen,XU Qing-lian,WANG Chang-rong (Burn Department,The First Affiliated Hospital of Anhui Medical University,Hefei,Anhui 230022,China);Clinical effects of ulinastatin in patients with severe burn sepsis[J];Anhui Medical and Pharmaceutical Journal;2010-11
3 XU Shi-jun,SHEN Ying-jun,JIN Shen-rui,XIE Yu-huan(Chendu university of TCM chengdu 610075);Efect of Ramulus Cinnamomi Volatile Oils on Expression of Toll-like Receptor2 and Receptor4 and MYD88 on Acute Pneumonia Model Induced by LPS[J];Journal of Chengdu University of Traditional Chinese Medicine;2008-03
4 Chen Xuxin,Wu Xueling,Chen Huaping,Qian Guisheng(Research Institute of Respiratory Diseases,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China);Effect of SIGIRR overexpression on activation of NF-κB induced by LPS in H292 cells[J];Chongqing Medicine;2011-08
5 ;Effect of ulinastatin on inflammation reaction and protection of lung during cardiopulmonary bypass in infants[J];Academic Journal of Second Military Medical University;2008-07
6 LIU Chang-hai1,2,SUN Shao-hua3,LI Hai-ling4,ZHANG Wei-hua51.Preclinical Medical College,Lanzhou University,Lanzhou 73000,China2.Department of Forensic Medicine,Inner Mongolian Medicine College,Hohhot 0100593.Pathological Institute,the First Hospital of Lanzhou University,Lanzhou 7300004.Department of Preventive Medicine,Inner Mongolian Medicine College,Hohhot 0100595.Aqi Public Security Bureau of Chifeng City,Chifeng 025571;Regulatory effect of ulinastatin on NF-κB and MMP-2/TIMP-2 in rats with acute renal trauma[J];Academic Journal of Second Military Medical University;2008-10
7 YANG Hong-guang,LI Feng,YOU Shu-hong,XU Fang-lin,ZOU Ting,LUO Yun(Department of ICU,the First People Hospital of Jiujiang,Jiujiang 332000,Jiangxi,China);Clinical observation of 53 cases with multiple organ dysfunction syndrome treated with ulinastatin combines with continuous veno-venous hemofiltration[J];Military Medical Journal of Southeast China;2008-03
8 LUO Zhen-chun1,HUANG Yan2,QIN Kai-xiu1,LING Zong-xiu1(1Department of Emergency,2Department of Anesthesiology,Second Affiliated Hospital,Chongqing Medical University,Chongqing 400010,China);Effect of ginkgolide B on NF-κB expression in pulmonary tissue of mice with acute lung injury[J];Acta Academiae Medicinae Militaris Tertiae;2009-14
9 XIONG Yi,WANG Xing-yong,ZHOU Hai-yin,HE Rong,DING Miao,QIU Chun-lan,ZHOU Jing,HE Yin ( Intensi Care Unit,Children’s Hospital,Chongqing Medical University,Chongqing 400014,China);Protective effects and mechanism of pyrrolidine dithiocarbamates on acute lung injury of LPS-induced septic rats[J];Acta Academiae Medicinae Militaris Tertiae;2009-21
10 LIN Sen, LUO Xiang-Xiang, LIU Song-Ping Department of Surgery, Hainan Provincial 3rd People's Hospital, Sanya 572000, China;Ulinastin in treatment of acute pancreatitis[J];Journal of the Fourth Military Medical University;2008-16
China Proceedings of conference Full-text Database 1 Hits
1 WEI Hong-xia~1 YANG Yi~2 QIU Hai-bo~2 GUO Tao~2 ZHAO Min-min~2 CHEN Qiu-hua~2. 1 Department of Infection,Nanjing Second Hospital,Southeast University,Nanjing 210003,China; 2 Department of Critical Care Medicine,Zhong-Da Hospital and School of Clinical Medicine,Southeast University,Nanjing 210009,China;Effects of the different fluids on alveolar epithelium barrier in rats with acute lung injury[A];[C];2008
【Co-references】
Chinese Journal Full-text Database 5 Hits
1 JIANG Long-yuan YANG Lian-hong CHANG Jian-xing HE Zhi-jiang YU Tao HUANG Zi-tong Department of Emergency,the Second Affiliated Hospital of Sun Yat-sen University,Guangzhou 510120,China;The effect of Ulinastatin on the delivery of cytokines in patients with septic shock[J];Chinese Journal of Emergency Medicine;2006-12
2 LI Xiu-jiang~1,DU Yu-jun~2,DONG Jun-shu~1,ZHANG Zhi-jun~3(1.Dept of Emergency Medicine;2.Dept of Kidney;3.Dept of Hematology,First Hospital,Jilin University,Changchun 130021,China);Protective effects of ulinastatin on injury of organs induced by endotoxin[J];Chinese Pharmacological Bulletin;2006-11
3 LIANG Xiao-zhong~1,YAO Hua-guo~2,ZHANG Yuan-Li~2.(1.GuangDong Medical College,Zhanjian,524001,China;2.ICU,The Affiliated Hospital of GuangDong Medical College,GuangDong 524001,China);Progress about Organ Protection of Ulinastatin in Sepsis[J];Medical Recapitulate;2006-16
4 ;大肠杆菌脂多糖对腹膜间皮细胞己糖激酶活性的影响[J];Chinese Journal of Pathophysiology;2006-11
5 SHAO Yiming, ZHANG Liangqing, DENG Liehua, YAO Huaguo. Department of Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong, China;Clinical study on effects of ulinastatin on patients with systemic inflammatory response syndrome[J];Chinese Critical Care Medicine;2005-04
【Secondary References】
Chinese Journal Full-text Database 1 Hits
1 HUO Aijing,MA Jianfei,LI Chengcheng,et al Department of Nephrology,The First Affiliated Hospital,China Medical University,Shenyang(110001);The Expression of Osteopontin in Rat Peritoneal Mesothelial Cells Stimulated by Lipopolysaccharide and the Protective Effect of Ulinastatin[J];Chinese Journal of Integrated Traditional and Western Nephrology;2011-11
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 CUI Hujun 1△ , ZHANG Mingli 1, XIAO Feng 1, LI Yan 1,WANG Jin 1, CHEN Hongyi 2 (1. Department of Cardiac surgery, 2. Department of Thoracic surgery, Peking University First Hospital, Beijing 100034, China);Comparison and correlative analysis of pulmonary function markers after extracorporeal circulation[J];Journal of Beijing Medical University;2003-02
2 LI Qi,QIAN Gui sheng,ZHANG Qing,GAO Zheng jin, ZHAO Qing,LI Hai bin (Institute of Respiratory Disease, Xingqiao Hospital,Third Military Medical University,Chongqing 400037,China);Study of acute lung injury induced by different doses of lipopolysaccharide in rats[J];Acta Academiae Medicine Militaris Tertiae;2001-11
3 Qian Guisheng. Insititute of Respiratory Disease, Xinqiao Hospital, Third Military Medical University, Chongqing 400037, China;THE STATUS QUO AND PROSPECT OF ACUTE LUNG INJURY AND ACUTE RESPIRATORY DISTRESS SYNDROME[J];Medical Journal of Chinese People's Liberation Army;2003-02
4 FANG Qiang, CHEN Pu.(Department of ICU,The First Affiliated Hospital Zhejiang University,School of Medicine,Hangzhou310003,China);A Study on clinical efficacy of ulinastatin in severe sepsis patients and its mechanism of action[J];Chinese Journal of Infection and Chemotherapy;2005-01
5 Peng Wan-yong,Cheng Yang-long,Peng Ai-fang,Zheng Qi-changDepartment of Trauma,Union Hospital,Tonji Medical College,Huazhong University of Science and Technology,Wuhan 430022;Experimental Study on the Role of Urinary Trypsin Inhibitor (UTI) in Pancreatic Wound-healing of the Rat[J];Chinese Journal of Clinical Gastroenterology;2003-04
6 ZHANG Hua-fang 1,LI Yi-zhao 2 ( 1Weifang People's Hospital,Weifang 261042,China; 2Shandong College Qilu Hospital);Experimental Study of Ulinastatin on the Protection of Cerebral Ischemia Injury[J];Acta Academiae Medicinae Weifang;2003-05
7 DENG Wen-feng,YU Li-xin,YU Yu-ming,et al.Organ Transplantation Center,Nanfang Hospital,Nanfang Medical University,Guangzhou 510515,China;Clinical study of ulinastatin in relieving the warm ischemia injury of renal graft[J];Chinese Journal of Urology;2004-11
8 YANG Lian-yue, YANG Zhi-li, WANG Wei, PENG Ji-xiang, HUANG Geng-wen. Department of General Surgery,Xiangya Hospital,Central South University, Changsha 410008,China;Protection of Ulinastatin on gut barrier in septic rats[J];Chinese Journal of General Surgery;2003-11
9 YU Yang, JIANG Shu-lin, LIU Yan-ping, et al. Department of Cardiovascular Surgery, The Second Affiliated Hospital of Harbin Medical University, Harbin 150086, China;The protective effect of tumor necrosis factor-αantibody on lung injury after cardiopulmonary bypass[J];Chinese Journal of Thoracic and Cardiovascular Surgery;2003-03
10 XU Kang-qing, SUN Pei-wu, HUANG Wen-qi, et al. Department of Anesthesiology, The First Affiliated Hospital, Sun Yet-sen University, Guangzhou 510080, China;Effects of ulinastatin on lung function after cardiopulmonary bypass[J];Chinese Journal of Thoracic and Cardiovascular Surgery;2004-01
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