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《Medical Journal of Chinese People's Liberation Army》 2011-10
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Effect of Toll-like receptor-4 and NF-κB on expression of matrix metalloprotease 9 induced by lipopolysaccharide in human airway epithelial cells

LI Ya-qing,XU Wu-lin,YAN Jian-ping,XIA Ying-jie,WANG Hong.Department of Respiratory Medicine,Zhejiang Provincial People's Hospital,Hangzhou 310014,China  
Objective To study the effects of toll-like receptor-4(TLR-4) and nuclear factor-κB(NF-κB) on the expression of matrix metalloprotease 9(MMP-9) induced by lipopolysaccharide(LPS) in human airway epithelial cells,and explore the influence of TLR-4 inhibitor TAK-242 on the expression of MMP-9.Methods In order to study the effects of LPS on MMP-9 expression,HBE4-E6/E7 cells were challenged for 24 hours by different concentrations of LPS(0,0.1,1,10,100 μg/L),or challenged for 0,8,12,16 and 24 hours by LPS at a concentration of 10 μg/L.HBE4-E6/E7 cells were pretreated for 30min with TAK-242(100μg/L) or PDTC(50mg/L),and challenged for 24h by LPS at 100 μg/L thereafter.The expression of MMP-9 mRNA was assessed by RT-PCR and of MMP-9 protein by Western blotting.MMP-9 activity was determined by gel zymography.The active NF-κB production was determined by electrophoretic mobility shift assay(EMSA).Results The expression of MMP-9 mRNA in HBE4-E6/E7 cells was induced by LPS in both dose-and time-dependent manner(P0.05).Both TAK-242 and PDTC significantly inhibited the expression of MMP-9 mRNA and protein(P0.05),down-regulated MMP-9 activity(P0.05),and significantly inhibited active NF-κB production induced by LPS(P0.05).Conclusion MMP-9 expression is induced by LPS in human airway epithelial cells via TLR-4/NF-κB signal pathway,which can be inhibited by TAK-242.
【Fund】: 浙江省医药卫生科学研究基金(No.2008B007)
【CateGory Index】: R563.9
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【Citations】
Chinese Journal Full-text Database 1 Hits
1 JIANG Jing-jing,SHI Xue-yin,YUAN Hong-bin,ZHU Qiu-feng,WANG Cheng-cai,SUN Hai-jing.Department of Anesthesiology,Changzheng Hospital,Second Military Medical University,Shanghai 200003,China;Effects of ulinastatin on NF-κB expression in lung tissue of rats with acute lung injury[J];Medical Journal of Chinese People's Liberation Army;2009-05
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 PENG Xia,MA Jianfei,LI Chengcheng,et alDepartment of Nephrology,The First Affiliated Hospital China Medical University,Shenyang (110001);Effect of Ulinastatin on Lipopolysaccharide-induced Interleukin 15,Interleukin 6 Expression in Rat Peritoneal Mesothelial Cells[J];Chinese Journal of Integrated Traditional and Western Nephrology;2010-10
2 CHEN Xiao-bing,XU Tie,LI Xiao-min,et al.Center of Emergency,First People's Hospital of Lianyungang,Affiliated Hospital Xuzhou Medical College,Lianyungang 222001,China;Explore the Effection and Machanism of Different Doses of Ulinastatin in Septic[J];Journal of Clinical Rational Drug Use;2011-29
【Secondary Citations】
Chinese Journal Full-text Database 5 Hits
1 LI Qi, QIAN Gui sheng, ZHANG Qing, WANG Chang zheng (Institute of Respiratory Diseases, Xingqiao Hospital, Third Military Medical University, Chongqing 400037, China);Effects of different doses of lipopolysaccharide on acute lung injury in rats[J];Acta Academiae Medicinae Militaris Tertiae;2004-10
2 QIAN Gui-sheng. Institute of Respiatory Disease of PLA,Xinqiao Hospital,Third Military Medical University,Chongqing 400037,China;Recent progress in research on the diagnosis and treatment of acute lung injury and acute respiratory distress syndrome[J];Medical Journal of Chinese People's Liberation Army;2009-04
3 Guo Zhenghui,Hong Xin,Mao Baoling,et al.Department of Instisute of Respiratory Disease,Xingqiao Hospital,Third Military Medical University,Chongqing 400037,China [;Effects of nuclear factor-κB activation on acute lung injury[J];Journal of Emergency Medicine;2003-09
4 SHEN Jin-mei, HE Xiao-jing, et al. Department of Anesthesiology, Second Hospital of Xiangya , Central South University, Changsha 410011, China;Effects of ulinastatin on cytoldnes and respiratory index during open heart surgery with CPB[J];Chinese Journal of Anesthesiology;2004-08
5 SHAO Yiming, ZHANG Liangqing, DENG Liehua, YAO Huaguo. Department of Intensive Care Unit, Affiliated Hospital of Guangdong Medical College, Zhanjiang 524001, Guangdong, China;Clinical study on effects of ulinastatin on patients with systemic inflammatory response syndrome[J];Chinese Critical Care Medicine;2005-04
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