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《Medical Journal of Chinese People's Liberation Army》 2012-06
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Effects of cholecystokinin-octapeptide (CCK-8) on iNOS expression and NO formation in rat alveolar macrophage after lipopolysaccharide challenge in vitro

WU Zong-sheng1,ZHENG Jia-yi1,YAO Yong-ming2,LU Zhong-qiu1 1Department of Emergency,First Affiliated Hospital of Wenzhou Medical College,Wenzhou,Zhejiang 325000,China 2Burns Institute,First Affiliated Hospital of General Hospital of PLA,Beijing 100048,China  
Objective To investigate the effects of cholecystokinin-octapeptide(CCK-8) on LPS-induced inducible nitric oxide synthase(iNOS) expression and nitric oxide(NO) secretion of rat alveolar macrophage cell line(NR8383).Methods NR8383 cells were cultured and pretreated with different concentrations of CCK-8(10-10,10-8 and 10-6mol/L) with or without 2μg/ml Proglumide(Pro) before LPS stimulation.The value of NO in the supernatant was measured by Griess reagent.The mRNA expression and activity of iNOS were determined by RT-PCR and biochemical detection,respectively.Results Compared with that of control group,LPS elevated the expression level of iNOS and the production of NO in rat alveolar macrophage cells significantly(P0.01).CCK-8 obviously inhibited LPS-induced iNOS expression and activation of NR8383 cells,reduced NO generation in a concentration-dependent manner(P0.05 or P0.01).Proglumide could reversed the inhibitory effect of CCK-8(P0.01).Conclusions CCK-8 effectively inhibit the LPS-induced NO production via inhibiting expression and activity of iNOS in rat alveolar macrophages.This might be the underlying mechanism of CCK-8 in alleviating endotoxic shock(ES) in an animal model.
【Fund】: 国家自然科学基金(81071593);; 浙江省医学扶植重点建设学科计划(07-F04)~~
【CateGory Index】: R459.7
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