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《Journal of Jinggangshan University(Natural Science)》 2012-05
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CONTENT DETERMINATION OF URSOLIC ACID IN TARAXACUM MONGOLICUM HAND.-MAZZ. FROM GUIZHOU BY HPLC

YANG Jin1, ZHU Jian-bo2, ZHANG Yi-han1 (1. School of Medicine, Hubei University for Nationalities, Enshi, Hubei 445000, China; 2. Minda Hospital of Hubei University for Nationalities, Enshi, Hubei 445000, China)  
Objective: To establish the content determination method of ursolic acid in Taraxacum mongolicum Hand.-Mazz by HPLC. Methods: Using HPLC to investigate the content determination. The HPLC system consisted of Dikma C18 (250 mm × 4.6 mm, 5 μm), mobile phase of methyl alcohol-acetonitrile-0.5% ammonium acetate (15:65:20) mixture, column temperature of 25℃ , flow rate of 1.0 mL/min, detection at 215 nm. Results: It was well linear between the concentrations from 10.2 to 102 μg/mL and the peak areas, the recovery rate was 99.52% (RSD = 1.47, n = 6). Conclusion:It is more suitable for ursolic acid as ground-level quality control for Taraxacum mongolicum.
【Fund】: 湖北省自然科学基金项目(2011CDC018);; 湖北省教育厅中青年科研项目(Q20122905)
【CateGory Index】: R284.1
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