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《ACTA LASER BIOLOGY SINICA》 2000-01
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Quick Preparation of Total DNA from Plants

WANG Yan 1 LI Shaoshan 2 LIU Songhao 2 (1 School of Life Science and Technology, Jinan University , Guangzhou 510632) (2.Institute of Laser Life Science, South China Normal University, Guangzhou 510631)  
A fast and simple way to isolate total DNA from up to 100mg of plant tissue was developed in the present paper. By using the DNeasy Plant Mini Kit, high quality of total DNA from plant was yielded in less than 1 hour. Purification required no phenol or chloroform extraction and involved minimal handling. This made the kit ideal for simultaneous processing of multiple samples. Plant DNA isolated with this method could be completely digested by restriction endonucleases, and was ready for use in all kinds of downstream applications including PCR, blotting, analysis of RAPD, AFLP and RFLP.
【Key Words】： DNA isolation rice (Oryza sativa) peanut (Arachis hypogaea) maize (Zay mays)
【Fund】： 广东省自然科学基金;; 广东省高教厅及广州市科委基础研究项目
【CateGory Index】： Q946.2
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【References】
Chinese Journal Full-text Database 10 Hits
1 Zhang Ning-jie~(1.2)(1 Agriculture College of Guangxi University,Nanning,530003;2 Key Laboratory of Guangxi Academy of Agricultural Science,Nanning,530003);Extracting High Quality DNA of Peanut[J];Anhui Agricultural Science Bulletin;2007-08
2 ZHAO Wei et al (College of Life Science and Technology,University of Electronic Science and Technology,Chengdu,Sichuan 610054);An Effective Method of DNA Extraction from Crassulaceae Plant[J];Journal of Anhui Agricultural Sciences;2006-22
3 ZHANG De-jun et al(Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences, Xining,Qinghai 810001);An High-effective Method of Extracting Genomic DNA from Saxifragaceae Plants[J];Journal of Anhui Agricultural Sciences;2008-16
4 Ming Jun;Zhang Qixiang;Yan Xiaolan;Qiu Lijuan. Department of Horticulture, Hubei Agricultural College, Jingzhou, 434025, P. R. China.;DNA template preparation and technology system building for AFLP analysis of Prunus mume.[J];Journal of Beijing Forestry University;2003-03
5 TIAN Zai-min1,2,GONG Xue-chen1,ZHANG Li-zeng3(1.College of Agriculture and Foresty,Hebei North University,Zhangjiakou 075131,Hebei,China;2.Beijing Research Center for Hybrid Wheat,Beijing Academy of Agricultural and Forestry Sciences,Beijing 100097,China;3.Agriculture Office of Zhangjiakou,Zhangjiakou 075000,Hebei,China);Comparison of Several Kinds of Wheat DNA Extraction Methods[J];Hubei Agricultural Sciences;2009-07
6 Yu Wenzheng Yang Gui Zhang Yingnan Huang Zhiyong (Agricultural Faculty,Guangdong Ocean University, Zhanjiang 524088);Study on the Extraction of Genomic DNA from Hot Pepper(Capsicum annuum L.)[J];Journal of China Capsicum;2007-04
7 ZHANG Rui,TANG Ming xia,WENG Zhou,WANG Li(College of Life Science,Sichuan University,Chengdu 610064,China);Application of AFLP Technique on the Genetic Diversity of Paris[J];Journal of Sichuan University(Natural Science Edition);2006-05
8 LIANG Xue-lian,ZHENG Yi-xiong,CHEN Xiao-ling,ZENG Jin-ji(Life Sciences College,Zhongkai University of Agricultural and Technology,Guangzhou 510225,China);Comparison of Different Methods of DNA Extraction of Peanut Leave[J];Biotechnology;2007-01
9 WU Juan,WU Jin-ping (1.Life Science Department,Anqing Teachers' College, Anqing 246011, China; 2.Economic Crops Institute of Hubei Academy of Agricultural Sciences, Wuhan 430064, China);The isolation of small amount DNA from rice[J];Journal of Biology;2007-04
10 MA Lei-lei1,XU Shi-chang2,CAO Yuan-yin1(1.Institute of Plant Immunology,Shenyang Agricultural University,Shenyang 110161,China;2.Institute of Plant Protection,Chinese Academy of Agricultural Sciences,Beijing 100094,China);A New Protocol for Fast DNA Extraction from Plant Seeds[J];Journal of Shenyang Agricultural University;2005-05
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 LIU Zun-chun et al(Henan Institute of Science and Technology,Xinxiang,Henan 453003);Comparison of Genomic DNA Extraction Methods of Jinhua Pear[J];Journal of Anhui Agricultural Sciences;2005-11
2 FANG Cong bing 1,SHENG Bin cheng 2,ZHANG Zhen 2 (1 Department of Horticulture,Anhui Agricultural University,Hefei 230036; 2 Department of Horticulture,Nanjing Agricultural University);Effect of Different Sample-making Methods for SEM on Pollen Morphology of Fruit Mei (Prunus mume Sieb et Zucc)[J];JOURNAL OF ANHUI AGRICULTURAL UNIVERSITY;2000-01
3 Zhao Changling 1, Guo Weiming 1, Chen Junyu 21. College of Life Sciences,Nanjing Agricultural University,210095,P. R. China; 2. School of Landscape Architecture,Beijing Forestry University,100083, P. R. China;Preliminary Aesthetical Analysis on the Beauty of the Flower Color of Prunus mume Sieb. et Zucc.[J];Journal of Beijing Forestry Unversity (Social Sciences);2003-04
4 Chen Junyu;A NEW SYSTEM OF CLASSIFICATION FOR CHINESE MEI FLOWER (PRUNUS MUME SIEB.ET ZUCC.) CULTIVARS[J];;1981-02
5 Zhang Qixiang;A COMPARATIVE STUDY ON DIFFERENCES IN COLD HARDINESS IN SOME OF MEI FLOWER CULTIYARS (PRUNUS MUME SIEB. ET. ZUCC)[J];;1985-02
6 Ma Yan Chen Junyu(Landscape Architecture Department, BFU);An Attempt to Evaluate Rose Blackspot Resistance of Some Parent Plants and Hybrid Seedlings During Breeding New Cultivars for Establishing Rejuvenation Rose Group[J];Journal of Beijing Forestry University;1992-03
7 Mao Hanshu Ma Yan Wang Zhongzhi(Beijing Forestry University);Studies on Numerical Classification of Chinese Mei Flower Cultivars[J];Journal of Beijing Forestry University;1992-04
8 Song Wan; Xu Jiuru. Journal of Beijing Forestry Universtity (2000)22(1)76～80 [Ch,24 ref.] College of Forest Resources and Environment, Beijing For. Univ., 100083, P. R. China.;Progress in application of germplasm identification and fingerprinting of fruit trees.[J];JOURNAL OF BEIJING FORESTRY UNIVERSITY;2000-01
9 Zhao Huien;Chen Junyu. Journal of Beijing Forestry University (2001)23(1)81～83[Ch,35 ref.] College of Landscape Architecture, Beijing For. Univ., 100083, P. R. China.;Some advances in molecular genetics of flower development and its potential value in breeding of ornamental plants.[J];Journal of Beijing Forestry University;2001-01
10 Zhou Chunling; Dai Silan. College of Landscape Architecture, Beijing For. Univ., 100083, P. R. China.;AFLP analysis of some Dendranthema spp.[J];Journal of Beijing Forestry University;2002-Z1
【Secondary References】
Chinese Journal Full-text Database 10 Hits
1 ZHOU He-feng et al (Department of bioengineering,Zunyi Medical College Zhuhai Campus,Zhuhai,Guangdong 519041);Study on Methods for Extraction of Genomic DNA from Rhizoma et Radix Notopterygii[J];Journal of Anhui Agricultural Sciences;2008-10
2 BU Yuan-yuan et al(Yanbian University Agriculture College Agricultural department,Longjing,Jilin 133400);Study on RAPD Reaction System for Endangered Pharmacy Plant Rhodiola Sachalinensis[J];Journal of Anhui Agricultural Sciences;2008-30
3 YUE Qing-ni et al(College of Life Sciences,Shihezi University,Shihezi,Xinjiang 832003);Genetic Diversity in Main Cultivars of Safflower in Xinjiang Uighur Autonomous Region Based on RAPD Analysis[J];Journal of Anhui Agricultural Sciences;2008-33
4 GAO Qing-bo et al (Key Laboratory of Adaptation and Evolution of Plateau Biota,Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai 810001);Preliminary Studies on ITS Sequences of nrDNA from Rhodiola alsia[J];Journal of Anhui Agricultural Sciences;2009-01
5 YANG Pei-hao et al(College of Life Science,Anhui Agricultural University,Hefei,Anhui 230036);Comparative Study on Extraction of Genomic DNA from Different Parts of Wheat[J];Journal of Anhui Agricultural Sciences;2009-22
6 TANG Jie,LIU Lian-sheng,XU Na-na,ZHAO Hai-quan(School of Life Science Anhui Agricultural University,Hefei 230036);Extraction methods of genomic DNA from Gynostemmna pentaphullum leaves[J];Journal of Anhui Agricultural University;2008-03
7 ZHU Hong-xia(Shanghai Polytechnic College of Urban Management,Shanghai 200438 China);DNA Template Preparation and Technology System Construction for AFLP Analysis in Paeonia suffruticosa[J];Northern Horticulture;2007-10
8 YANG Li,WANG Yu-zhu,SUN Hao-yuan,ZHANG Jun-huan(Institute of Forestry & Pomology,Beijing Academy of Agriculture & Forestry Sciences,Beijing 100093,China);Extraction of Almond Genomic DNA[J];Northern Horticulture;2008-02
9 GUI Teng-qin1,2,3,QIAO Ai-min3,SUN Min1,WANG Xin-yan3,SUN Xue-mei1(1.College of Life Science,Southwest University,Chongqing 400715,China;2.Dept.of Chemistry & Life Science,Qianxinan Teachers' College,Xingyi,Guizhou 562400,China;3.College of Agriculture and Landscape Architecture,ZhongKai University of Agriculture and Technology,Guangzhou,Guangdong 510225,China);Comparison of Extraction Methods and ISSR Analysis of Genomic DNA in Japanese apricot(Prunus mume Sieb.et Zucc.)[J];Northern Horticulture;2008-04
10 LI Chun-xia,LI Hong-fei(Yan-an Vocational and Technical College,Yanan,Shanxi 716000,China);Study on Efficient Extraction for Genomic DNA from Malus Leaves by CTAB Method[J];Northern Horticulture;2009-02
China Proceedings of conference Full-text Database 1 Hits
1 Qi Jing Shen Lianying Mao Yongmin Song Renping (College of Horticulture,Agriculture University of Hebe,Baoding 071001,China);Research advances on molecular markers in Chinese Jujube[A];[C];2005
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