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《Acta Laser Biology Sinica》 2004-05
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Extraction and Purification of the Pigment of Natural Brown Cotton and its UV Spectral Properties

ZHAN Shao-hua~(1,2),LIN Yi~(1*),CAI Yong-ping~1, WANG Shu~1 (1. Life Science School,Anhui Agriculture University,Hefei 230036,Anhui,China; 2. Biology Department,Wanxi University,Wanxi 237012,Anhui,China)  
In the procession of extracting the pigment of natural brown cotton,16 kinds of chemicals were selected as the solvents to dissolve the fiber of natural brown cotton,of which both the distilled water and NaOH(pH=9) were the most effective and economical. On this basis,the pigment of natural brown cotton was extracted and purified,and it's UV spectrum and chemical characters were also analyzed. Our results indicated Ethanol- precipitation method,re-crystallization-method,copper or lead salt-precipitate method,paper chromatography method and column chromatography were practical purification methods of the pigment. Analyzing the UV spectrum of natural brown cotton,there were strong absorbed peak near in 218 nm and weak absorbed peak near 264 nm,which suggests double bands and phenol hydroxyl existed in the chemical structure of the pigment.
【Fund】: 国家科技攻关计划子课题;离子束应用技术研究(2001BA302B 03)
【CateGory Index】: TQ28
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