Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Laser Biology Sinica》 2006-01
Add to Favorite Get Latest Update

Studies on Introduction of Antifungal Genes into Cotton Plants by Laser Microbeam Puncture

CHEN Ling-na~1,QU Yan-ying~1,WANG Lan-lan~2,ZHANG Jinwen~(2,3*),LIU Gui-zhen~2,CHEN Zheng-hua~2(1.Agronomy College,Xinjiang Agricultural University,Urumqi 830052,Xinjiang;2.Postdoctoral Scientific Research Station of Gansu Yasheng Industrial(Group) Co.Ltd,Beijing Branch,Beijing 100101;3.Agronomy Department,Gansu Agricultural University,Lanzhou 730070,Gansu)  
Using cotton susceptive seed-germinating embryos as transgenic receptor,the bivalent genes pBIBGC,which includes β-1,3-glucanase gene and chitinase gene,was introduced into cotton by laser microbeam Puncture Techniques(LMPT).There are npt-Ⅱ(neomycin phosphotransferase Ⅱ) gene as screening marker in the plant bivalent expression vector.From treated embryos by laser transformation,resistant plantlets have been obtained after kanamycin resistance screening.In this research,we have also studied some key factors that influenced LMPT,such as the duration of pretreatment of susceptive seed-germinating embryos and hypertonic treatment,and so on.The results indicated that the LMPT was easy to operate and repetitive.The foreign genes were introduced into susceptive seed-germinating embryos by this technique,which avoids difficulties of regeneration of plantlets.
【CateGory Index】: S562
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 4 Hits
1 DUAN Hong-ying,DING Xiao-sheng(The College of Life Science,Henan Normal University,Xinxiang 453007,China);Cloning of Arabidopsis thaliana DREB2A Gene and Its Construction of Its Plant Fluorescent Expression Vector[J];Acta Agriculturae Boreali-Sinica;2008-03
2 ZHENG Ju-yun1,CHEN Zheng-hua2,LIU Gui-zhen2,SU Xiu-juan2,QU Yan-ying1(1.Agronomy College,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;2.Postdoctoral Scientific Research Station of Gansu Yasheng Industrial Co.Ltd,Beijing Branch,Beijing 100101,China);Construction of Expression Vector with Anti-insect Bivalent Gene and Study on Transformation Plant by Laser Microbeam[J];Acta Laser Biology Sinica;2008-04
3 WANG Bao-zhen,LI Shu-ting(Physics and Electronic Information Engineering Department of Shangluo University,Shangluo,Shaanxi 726000);Laser Induced Plant Organization:the New Progress[J];Journal of Shangluo University;2010-04
4 WANG Shou-yun 1,2,WANG Yun-peng 1,3,XING Shao-chen 2,GUAN Shu-yan 1,et al.(1.School of Life Sciences,Jilin Agricultural University,Changchun 130118; 2.Agro-Biotechnology Research Institute,Jilin Academy of Agricultural Sciences,Changchun 130033; 3.Faculty of Agronomy,Jilin Agricultural University,Changchun 130118,China);Development of Agrobacterium-mediated Transformation System Using Germinating Embryo as Explants and Generation of Herbicide Resistant Transgenic Plants in Maize[J];Journal of Maize Sciences;2013-02
【Citations】
Chinese Journal Full-text Database 4 Hits
1 Fu Guirong Su Huanran Huang Yongfen Wang Qingyin(Harbin Normal University);STUDIES ON TRANSFORMATION OFSEED-GERMINATING· EMBRYOES OF SOYBEANBY A. TUMEFACIENS MEDIATED IPT GENE.Ⅲ Analysis of the shape characteristics of the seedling stage[J];NATURAL SCIENCE JOURNAL OF HARBIN NORMAL UNIVERSITY;1999-02
2 Fu Daolin Wang Lanlan (Institute of Genetics,Chinese Academy of Sciences,Beijing 100101);The Developments about Laser Microbeam Transgenic Techniques[J];ACTA LASER BIOLOGY SINICA;1999-01
3 Liu Guizhen 1 Lan Haiyan 2 Tian Yingchuan 3 Li Shucheng 4 Le Jinhua 5 Wang Lanlan 1 Chen Zhenghua 1 1Institute of Genetics, The Chinese Academy of Sciences, Beijing 100101 2Institute of Industrial Crops, Xinjiang Academy of Agri;Studies on Verticillium and Fusarium Wilt Resistant Cotton Plants with Trangenosis by Laser Microbeam Puncture[J];CHINESE JOURNAL OF LASERS;2000-03
4 Xu Yao, Wang Ting and Li Bao-jan (Biotechnology Research Centre. Zhongshan University. Guangzhou 510275);STUDIES ON TRANSFORMATION OF SEED-GERMINATING EMBRYOES OF PLANTS BY AGROBACTERIUM-MEDIATED FOREIGN GENES[J];Acta Biologiae Experimentalis Sinica;1991-02
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YANG Li-heng et al (College of Agronomy,Xinjiang Agricultural University,Urumuqi,Xinjiang 830052);Study on the Transformation Method of Mediating Exogenous Gene into Cotton Epicotyl by Agrobacterium[J];Journal of Anhui Agricultural Sciences;2008-19
2 WANG Ting-pu et al(Department of Life Science and Chemistry,Tianshui Normal University,Tianshui,Gansu 741000);Research Advance of β-1,3-glucanase and Chitinase in Controlling Insect Pests of Crops[J];Journal of Anhui Agricultural Sciences;2010-26
3 FENG Lu~1,NA Ri~2(1.Department of Applied Physics,College of Science,Beijing Science and Engineering University,Beijing 100081,China;2.Department of Physics,College of Science and Engineering,Neimongol University,Hohhot 010021,China);Physical methods in plant breeding[J];Pratacultural Science;2005-12
4 XU Kai-jie,YAN Ming-xian,CHAI Guai-qiang,SUN Feng-li,LIU Shu-dong,XI Ya-jun(College of Agriculture,Northwest Agriculture and Forestry University,Yangling 712100,China);Switchgrass seeds germination and seedling growth and development with different concentration of Agrobacterium tumefaciens[J];Acta Prataculturae Sinica;2012-01
5 HAN Ya-ping1,CAO Yan1,CHEN Bing-cai2(1Department of Physics,College of Science,Northeast Forestry University,Harbin 150040,Heilongjiang;2College of Computer Science and Technology,Harbin Engineering University,Harbin 150001,Heilongjiang,China);Development Trend of Laser Mutation Breeding for Soybean[J];Soybean Science;2008-03
6 Wang Qingyin;Huang Yongfen;Fu Guirong;Xu Zhiru;cheng Baojiang;Li HOngquan (Department of Biology,Harbin Normal University);STUDIES ON TRANSFORMATION OF SEED-GERMINATING EMBRYOES OF SOYBEAN BY Y.tumefaciens MEDIATED IPT GENE─ⅠAnalysis of the Isozymes of the Seedling Stage Cotyledons[J];SOYBEAN SCIENCE;1994-02
7 XU Kai-jie,SHI Li-li,LIU Shu-dong,XI Ya-jun(College of Agronomy,Northwest A&F University,Yangling,Shaanxi 712100,China);Wheat Seed Germination and Seedling Physio-biochemical Characteristics with Co-cultivation Conditions during Agrobacteriumtume faciens Soaking[J];Acta Botanica Boreali-Occidentalia Sinica;2011-04
8 ZHANG Zhi-zhong,WU Jing-hua,Lü Liu-xin,LIN Yi-zhang(College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China);Progress of plant chitinase and its application[J];;2005-04
9 CHEN Ai-kui,ZHUANG Wen-song,CHEN Dong-mei(Department of Biology,Guangdong Education Institute,Guangzhou,Guangdong,510303,P.R.China);Advances in the Research on Genetic Engineering of Plant Chitinase[J];Journal of Guangdong Education Institute;2010-03
10 Shao Hongbo(Guangxi Institute of Botany,Yanshan,Guilin,Guangxi,541006,China);An Outlook on the Developmental Research of Transgenic Plant Drug[J];Guangxi Sciences;2002-01
China Proceedings of conference Full-text Database 1 Hits
1 QI Jun-sheng WANG Qi MA Cun ZHANG Yuan-en ( Department of Plant Pathology, College of Agronomy and Biotechnology, China Agricultural University, Beijing 100094, China; Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100094, China);Progress on Molecular Genetics and Breeding Research of Cotton Verticillium Wilt Resistance[A];[C];2005
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 QI Zu-min et al(College of Opto-electronic Science and Technology,National University of Defense Technology,Changsha,Hunan 410073);Effects of Water and Narcissus Irradiation by Laser on the Growth of Narcissus[J];Journal of Anhui Agricultural Sciences;2008-35
2 ZHANG Jun et al(College of Life Science,Northwest University,Xian,Shaanxi 710069);Mutagenic Effect of YAG Laser on Antagonistic Bacteria WB7 against Alternaria solani[J];Journal of Anhui Agricultural Sciences;2008-36
3 HAN Ya-ping1,CAO Yan1,CHEN Bing-cai2(1Department of Physics,College of Science,Northeast Forestry University,Harbin 150040,Heilongjiang;2College of Computer Science and Technology,Harbin Engineering University,Harbin 150001,Heilongjiang,China);Development Trend of Laser Mutation Breeding for Soybean[J];Soybean Science;2008-03
4 CHEN Huo-ying~1,ZHANG Jian-hua~2,ZHUANG Tian-ming~1,ZHANG Xiao-ning~3 (1 Department of Plant Science,Shanghai Jiaotong University,Shanghai 201101,China;2 Department of Gardens of Commerce,Shanghai Commercial Polytechnic College,Shty,Shanghai 201101,China);Developing new germplasms of Lycopersicon esculentum for salt tolerance by pollen tube method[J];Acta Botanica Boreali-occidentalia Sinica;2004-01
5 LIU Chuan-liang,WU Zhi-xia,ZHANG Chao-jun,LI Feng-lian,WANG Yu-fen,LI Fu-guang (Chinese Cotton Research Institute, Chinese Agricultural Academy of Sciences; Key Laboratory of Cotton Genetic Improvement, Ministry of Agriculture,Anyang,Henan 455112,China);Study on large-scale and high efficient transformation system mediated by Agrobacterium tumefaciens of cotton[J];Acta Botanica Boreali-occidentalia Sinica;2004-05
6 ZHANG Lin-shui 1,ZHU Zhen 2,WU Xia 1,JIANG Yan-li 1,XU Hong-lin 2, SHANGGUAN Xiao-xia 1,LI Bo 1,LI Yan-e 1* (1 Cotton Research Institute, Shanxi Academy of Agricultural Sciences, Yuncheng,Shanxi 044000,China;2 Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101,China);Acquisition of Transgenic Colour Cotton with a Trivalent Insect-resistant Gene[J];Acta Botanica Boreali-occidentalia Sinica;2005-06
7 GUI Guang-rong,LIU Zheng,ZHANG Zi-xue,ZHANG Cong-yu(Plant Science Department of Anhui Science and Technology University,Fengyang,Anhui 233100,China);Construction of the Regeneration System for In Vitro Culture of Oppositifolious Maize[J];Acta Botanica Boreali-occidentalia Sinica;2005-12
8 DU Jian-zhong,SUN Yi,HAO Yao-shan,WANG Yi-xue,WANG Chang-biao,WANG Ming (Biotechnology Research Center,Shanxi Academy of Agricultural Sciences,Taiyuan 030031 China);Production of Herbicide-resistant Plants Transformed by Bar Gene and Their Basta-tolerance Activity[J];Acta Botanica Boreali-Occidentalia Sinica;2012-02
9 Sun Guoqing Li Xueyuan * Qin Wenbin Mo Ming Tuerxunjiang Ai Xiantao Economic Crop Research Institute, Xinjiang Academy of Agricultural Sciences, Urumqi,830000;The Research Status of Transgenic Cotton Breeding in Xinjiang[J];Molecular Plant Breeding;2004-01
10 Wu Rui Zhang Shuzhen*Institution of Tropical Bioscience and Biotechnology CATAS, Haikou, 571101;Green Fluorescent Protein and Its Application in Plant Molecular Biology[J];Molecular Plant Breeding;2005-02
【Secondary References】
Chinese Journal Full-text Database 5 Hits
1 HAO Feng1,LIU Xiao-jing1*,MAO Juan2,YU Tie-feng1,ZHANG De-gang1(1.Pratacultural College,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China;2.College of Agronomy,Gansu Agricultural University,Lanzhou,Gansu Province 730070,China);Construction of Antifreeze Protein Gene AFP Expression Vector and Transformation into Alfalfa Callus[J];Acta Agrestia Sinica;2009-06
2 LIU Xiao-jing1,2,HAO Feng1,2,ZHANG De-gang1,2,MAO Juan3,YU Tie-feng1,2(1.College of Grassland Science,Gansu Agricultural University,Lanzhou 730070,China;2.Key Laboratory of Grassland Ecosystem of Ministry of Education,Sino-U.S.Centers for Grazing land Ecosystem Sustainability,Lanzhou 730070,China;3.College of Agronomy,Gansu Agricultural University,Lanzhou 730070,China);Construction of antifreeze gene CBF_2 expression vector and transformation into alfalfa callus[J];Acta Prataculturae Sinica;2011-02
3 WANG Yun,REN Yong-bing,YANG Shuo,HAN Yu,TAO Yang,CAO Shu-qing (School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China);Cloning of Arabidopsis thaliana AtMYB61 gene and the expression vector construction[J];Journal of Hefei University of Technology(Natural Science);2011-05
4 L Shen-chao,WANG Hai-tao,YU Bin,MENG Wei-wei,CAO Shu-qing(School of Biotechnology and Food Engineering,Hefei University of Technology,Hefei 230009,China);Cloning of Arabidopsis HIS1-3 gene and construction of its expression vector[J];Journal of Hefei University of Technology(Natural Science);2012-01
5 TU Shiwei1,GUO Wanli2,JIANG Lixi2,PAN Jianwei1(1.College of Chemistry and Life Science,Zhejiang Normal University,Jinhua Zhejiang 321004,China;2.Department of Crop Science,College of Agricuture and Biotechnology,Zhejiang University,Hangzhou Zhejiang 310012,China);Progress of gene transformation methods in rapeseed[J];Journal of Zhejiang Normal University(Natural Sciences);2012-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Li Hongquan;Li Hongwei;Wang Qian;Huang Yongfeng;Wang Qingyin(Harbin Normal University,Harbin,150080);STUDY ON TUMOR-INDUCED ERACTION OF CHINESE CULTIVAR SOYBEAN INFECTED BY Agrobacterium Tumefaciens CHRY5[J];SOYBEAN SCIENCE;1994-02
2 Fu Guirong; Xu Zhiru; Zheng Baojiang;Huang Yongfen;Wang Qingyin; Li Hongquan(Harbin Normal University);STUDIES ON TRANSFORMATION OF SEED-GERMINATING EMBRYOES OF SOYBEAN BY A.TUMEFACIENS MEDIATED IPT GENE ⅡAnalysis of the Isozymes of the Seedling Stage Roots[J];NATURAL SCIENCE JOURNAL OF HARBIN NORMAL UNIVERSITY;1996-04
3 ZHOU Miaoping, LU Weizhong (Institute of Agrobiological Genetics and Physiology, Jiangsu Academy of Agricultural Sciences, Nanjing210014);Plant Chitinases and Its Application in Anti fungal Gene Engineering[J];JIANGSU JOURNAL OF AGRICULTURAL SCIENCES;1998-03
4 Xu Yao;Jia Jingfen;Cheng Kuochang;Studies on Genetic Transformation of Callus Cultures of Hyoscyamus muticus+Nicotiana tabacum Somatic Cell Hybrids[J];Journal of Lanzhou University;1988-03
5 Jiang Hao Qin Hongmin Tian Yingchuan (Institute of Microbiology, Academia Sinica\ Beijing100080) Dean W. Gabriel (Plant Pathology Dept. University of Florida. Gainesville FL 32611, USA);CLONING OF A PROMOTER FRAGMENT OF BARK STORAGE PROTEIN GENE FROM POPULUS DELTOIDES AND ITS FUNCTION IN TRANSGENIC TOBACCO PLANTS[J];SCIENTIA SILVAE SINICAE;1999-05
6 Yu Hanshou 1,Zhang Yimin 2,Chen Yongxuan 1 and Wu Hanzhang 3 ( 1 College of Resources and Environmental Sciences, 2 College of Sciences, Nanjing Agric Univ,Nanjing 210095; 3 Zhenjiang Institute of Agricultural Sciences,Jurong City, Jiangsu Prov;Rape chitinase purification and possible function in resistance to rape root rot( Sclerotinia sclerotiorum )[J];JOURNAL OF NANJING AGRICULTURAL UNIVERSITY;1999-03
7 Chen Chongshun 1,Zhu Xuefeng 1 and Yu Zhifang 2 ( 1 Dept of Horticulture, 2College of Food Science and Technology, Nanjing Agric Univ,Nanjing 210095);Chitinase activities from different cultivars of Vigna sesquipedalis and their effect on the growth of Fusarium oxysporum f.niveum[J];JOURNAL OF NANJING AGRICULTURAL UNIVERSITY;1999-04
8 Wang Shoucai\ Wang Guoying\ Dai Jingrui (Agriculture university of China Zip Code: 100094);Discussion for Questions on Transgenic Genetics of Plant[J];PROGRESS IN BIOTECHNOLOGY;2000-04
9 Zeng Yan\ Zhao Nanming\ Liu Jinyuan (School of Life Science and Engineering Tsinghua University,Beijing 100084);Chitinases and Plant Defence Reactions[J];PROGRESS IN BIOTECHNOLOGY;1997-04
10 Gao Bi da (Department of Plant Protection, HNAU, Changsha\ 410128);Strategy of Chitinase Gene Transfer for Plant Disease Control: Progress, Problems and Prospect[J];PROGRESS IN BIOTECHNOLOGY;1999-02
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved