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Monitoring the Dynamics of Caspase-3 Activation Induced by Xiaoaiping

CHEN Tong-sheng,WANG Long-xiang,SUN Lei,WANG Hui-ying, XING Da(MOE Key Libratory of Laser Life Science & Institute of Laser Life Science,South China Normal University,Guangzhou 510631,Guangdong,China)  
Xiaoaiping(XAP) isolated from the stems of Marsdenia tenacissima,an Asclepiadaceae plant,has been documented to possess functions against inflammation and cancers.In this paper,we used the CCK-8 to measure the cell activity after XAP treatment according to the 1 ∶1 of XAP and DMEM,and we found that XAP inhibited obviously the cell activity.We used confocal fluorescence microscope and fluorescence resonance energy transfer(FRET) to measure the dynamic temporal-spatial distribution of SCAT3 and caspase-3 activation in single living human lung adenocarcinoma line(ASTC-a-1) cell expressing stably with the SCAT3.Experimental results showed that SCAT3 translocated from cytoplasm to nuclear,cell membrane and intracellular membrane area after 20 min of XAP treaument,and the caspase-3 was activated rapidly after about 100 min of XAP treatment.
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