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《Acta Laser Biology Sinica》 2008-04
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Construction of Expression Vector with Anti-insect Bivalent Gene and Study on Transformation Plant by Laser Microbeam

ZHENG Ju-yun1,CHEN Zheng-hua2,LIU Gui-zhen2,SU Xiu-juan2,QU Yan-ying1(1.Agronomy College,Xinjiang Agricultural University,Urumqi 830052,Xinjiang,China;2.Postdoctoral Scientific Research Station of Gansu Yasheng Industrial Co.Ltd,Beijing Branch,Beijing 100101,China)  
Bacillus thuringinesis gene and Amaranthus retroflexus agglutinin gene were ligated into bivalent plant expression vector,which can encode a new lepidopter-resistance and coleopterous insect-resistance protein.The bivalent gene(Bt-BA) were ligated into prokaryotic expression vector(pET28a) and plant expression vector(pBI121).These recombinant plasmids pET-BA and pBI-Bt-BA were confirmed by restriction enzyme and PCR analysis and the results showed that these recombinant plasmids were constructed successfully.The bivalent gene was induced into cole,and the transgenic cole has been obtained.
【CateGory Index】: S565.4
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