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《Acta Laser Biology Sinica》 2008-04
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Purification and Characterization of Cellulases from Humicola insolens

WANG Zai-gui1,HE Rui-guo2,ZHANG Hong-fu3,QIAN Shi-jun4,LIU Chao-liang1(1.College of Life Science,Anhui Agriculture University,Hefei 230031,Anhui,China;2.College of Animal Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China;3.Institute of Animal Sciences,Chinese Academy of Agriculture Sciences,Beijing 100094,China;4.Institute of Microbiology,Chinese Academy of Sciences,Beijing 100080,China)  
The cellulases from Humicola insolens,which has high ability to synthesze high stability cellulases,were purified by means of 1.5 folds volume ethanol deposition,DEAE-SephadexA-50 chromatography and SephadexG-100 chromatography.The purified cellulase showed a single band on SDS-polyacrylamide gel electrophoresis(PAGE).The biocatalyzed characters of the purified cellulase were investigated with the CMC-Na as substrate.The most appropriate catalyzed temprature and pH for it is 60 ℃ and 5.5 respectively.It has the extreme stability at the temperatures no more than 50 ℃.The CMC-Na has the protective effect on the cellulase.The pH stability range of the purified cellulase is 4 ~ 8.Zn2+,Ca2+,Mg2+,K+,Li+ can ativate the cellulase.And cellulase can mainly decompose CMC and salicin,but can decompose other substrates feebly.
【Fund】: 国家自然科学基金项目(30371088);; 合肥市应用技术与开发项目[合科(2008)26-44]
【CateGory Index】: Q814
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