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《Journal of Yangtze University(Natural Science Edition)Medicine V》 2008-03
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Influence on the Subpopulations of T Lymphocytes in Mice Administrated FHZ

SHI Ping(Department of Pharmacology,Hubei College of Chinese Medicine,Jingzhou 434000,China)  
Objective:To study the influence on the subpopulations of T lymphocyte in mice administrated Fu fang hu zhang jiao nang(FHZ).Methods:Adopt 10% xanthine i.p.in mice to duplicate hyperuricemia model,and administrate drugs for 6 days beforehand.Then,to detect serum urate concentration by uricase chromatometry and the percent of CD+3、CD+4、CD+8 and CD+4/CD+8 ratio of spleen T lymphocytes by monochromic flow cytometry(FCM).Results:5.0%,2.5%,1.25% FHZ group and allopurinol group had significant differentiation respectively compared with model group(P0.01,n =8),and were lower than model group,respectively.And 5.0%,2.5% FHZ group had significant differentiation respectively compared with allopurinol group.It denoted that different dosage FHZ can respectively reduce serum urine content in hyperuricemia of mice,and even be lower than normal level.In contrast,there were no significant differences between allopurinol group,model group and normal group in the percentage of CD+3、CD+4、CD+8 cells and CD+4/CD+8 ratio in spleen lymphocytes,nor were there significant differences between normal group,model group,allopurinol group and 5% FHZ group in the percent of CD+3、CD+4、CD+8 cells or CD+4/CD+8 ratio of spleen lymphocytes.Conclusion:FHZ can obviously reduce serum urine concentration.But acute hyperuricemia in mice and FHZ do not have significant influence in spleen T lymphocyte subpopulations
【CateGory Index】: R285
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