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Screening and Application of Functional Bacteria in the Advanced Treatment Process of Landfill Leachate

JI Fang-ying1,XIE Zhi-gang2,QIU Xue-min1,HUANG He1,ZHANG Lin-yuan1(1.Key Laboratory of Three Gorges Reservoir Region's Eco-Environment(Ministry of Education),Chongqing University,Chongqing 400045,P.R.China;2.Chongqing University of Arts & Sciences,Chongqing 402160,P.R.China)  
To improve organic pollutant degradability of landfill leachate,culture medium was prepared with landfill leachate and agar.And then three functional bacteria were cultured and screened from sludge in aeration pool of biochemical treatment system by bioaugmentation.Based on 16S rDNA identification,these bacteria were named Mari-nobacter(coded Y1),Acinetobacter(coded Y2) and Escherichia(coded Y3).Functional bacteria were scaling up,and then inoculated into biological-activated carbon(BAC) reactor by physical circle absorption method.With the control experiment,it was shown that the natural start-up BAC had significant degradation capacity to organic pollutants with molecular weight M of 10 ~ 5 kDa.And the inoculums' start-up BAC with functional bacteria was kept with was with removal rate of 76.1% for organic pollutants with molecular weight M of 100 ~ 30 kDa,and 80.9% for organic pollutants with molecular weight M exceeding 100 kDa.
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