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《Journal of Economic Animal》 2005-03
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Cloning and Expressing of out Membrane Protein OMP2b from Brucella ovis in Xinjiang

TENG Wen-jun,CHEN Chuang-fu,REN Xue-yan,WANG Yuan-zhi,YANG Li-juan,BAO Hui-fang,LIU Wen-jin (Laboratory of Xinjiang Endemic & Ethnic Diseases,Shihezi 832003,China)  
Objective: to express OMP2b antigen protein of OMP36 from out membrane protein of Brucella ovis in XinJiang and study the probability for it as molecular bacterin and diagnose antigen.Methods : OMP2b protein gene fragment was amplified from genomic DNA of Brucella ovis in XinJiang through PCR.The fragment was identified and cloned into prokaryotic expression vector PET-28a(+).Then IPTG was used to induce expression of OMP2b antigen protein.Result:(1)An about 1 089bp length PCR product was obtained and the sequence was the same as OMP2b of B.ovis sequence reported before.(2)An expression band about 36 ku was found.Conclusion: we obtained OMP2b gene fragment and expressed successfully in Escherichia coli.
【Fund】: 新疆兵团博士基金(兵博02)
【CateGory Index】: S852.61;
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