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《Journal of Economic Animal》 2013-01
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Analysis of Simple Sequence Repeats(SSR) Information in Expressed Sequence Tag(EST) Resources of Pelodiscus sinensis

XU Xiao-jun,ZHANG Hai-qi,ZHANG Chao,HE Zhong-yang(Zhejiang Fishery Technology Extention Station,Hangzhou 310012,China)  
A total of 4324 Pelodiscus sinensis ESTs,178 obtained from NCBI database and 4 146 from sequencing cDNA library of ulcer disease infected liver,were analyzed.And 2 644 non-reduntant ESTs with total length about 1 630.26 kb were obtained.One hundred ninty-seven EST-SSRs distributed in 137 ESTs were mined out by MISA.The frequency of these EST-SSRs was 7.45% and the mean distance of distribution was 8.28 kb in non-redundant ESTs.Dinucleotide and trinucleotide repeats were the main types in Pelodiscus sinensis,accouting for 60.91% and 35.53% of all the SSRs,respectively.AC/GT and AGC/CTG were the most frequent motifs,accounting for 44.17% and 62.86% in dinucleotide and trinucleotide repeats,respectively.The results showed that EST-SSRs and types of tandem repeats were rich in the Pelodiscus sinensis ESTs,and it was applicable to develop SSRs from its EST resources.
【Fund】: 浙江省水产农业新品种选育重大科技专项(2012C12907-1)
【CateGory Index】: S966.5
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