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《Journal of Emergenoy Medicine》 2003-01
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The role of nuclear factor kappa B activation in rats with acute lung injury

QIU Haibo,YANG Yi,ZHOU Shaoxia,et al.Department of Critical Care Medicine,Zhongda Hospital and School of Clinical Medicine,Southeast University,Nanjing 210009,China  
Objective To investigate the potential role of nuclear factor kappa B (NF κB) activation in rats with acute lung injury(ALI) induced by lipopolysaccharide(LPS).Methods ALI murine model was induced by infusing LPS intraperitoneally.At 0h,1h,3h,6h,and 12h after LPS exposure,lung wet/dry weight ratios(W/D) was recorded to assess lung injury.The total lung homogenates were prepared to detect NF κB activity by EMSA,tumor necrosis factor(TNF) α and interleukin(IL) 10 levels by ELISA,and mRNA expression by RT PCR.Results After LPS injection,W/D was increased obviously at 3h(4 80±0 31)and peaked at 6h(4 82±0 10),which were higher than W/D at 0h(3 67±1 04).NF κB activity in lung homogenates increased markedly from(44 8±30 9)at 0h to(405 7±62 4)at 6h.The level of TNF α in lung homogenates increased significantly at 6h (197 1±52 4)pg/ml,compared with pre LPS injection(61 2±10 7 pg/ml).IL 10 peaked at 12h after LPS injection(164 9±39 7)pg/ml,which was higher than the level of IL 10 at pre LPS exposure(71 6±15 9)pg/ml.Compared with pre LPS level,TNF α and IL 10 mRNA expression in lung homogenates increased obviously at 3h to 12h after LPS injection.Histologically,massive alveolar edema,hemorrhage,and inflammatory cell infiltration were observed.Conclusion LPS could induce NF κB activation and up regulate inflammatory mediator expression in ALI rats.The activation of NF κB and increased expression of cytokines might play an important role in the pathogenesis of ALI.[
【Fund】: 江苏省青年基金资助项目 (BQ980 3 5 ) ;; 铁道部科学基金资助项目 (J98Z0 2 6) ;; 东南大学科学基金资助项目 (92 473 41174)
【CateGory Index】: R563.9
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