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《Journal of Jilin University (Science Edition)》 2005-05
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Extraction of Acanthopanacis Senticosi Polysaccharides and Their Antioxidative Effect

MENG Qing-fan,YU Xiao-kun,XU Mu-yun,LI Ming-liang, GAO Zhi-hui,FAN Hao,CHU Yu-zhuo,TENG Li-rong(College of Life Science,Jilin University,Changchun 130012,China)  
Acanthopanacis Senticosi Polysaccharides(ASPs) were extracted by hot water.ASPs in the filtrate were successively precipitated with alcohol.When the quantity of alcohol was equal to the volume of the ASPs(filtrate,) the production yield of ASPs was the highest.The yields of the fraction productions were decreasing with the increase of the quantitiy of alcohol and the total yield of ASPs was up to(0.54%.) The inhibitions of the fraction productions to(O~-_2) and ·OH were assayed by the method of pyrogallol in the fenton system.The results show that the antioxidative effect of the fraction productions is improved with the increase of the quantity of alcohol.When the quantity of alcohol was 6 times the volume of ASPs filtrate,the elimination rate of the production to(O~-_2)could reach to the highest value of(47.7%.) When the quantity of alcohol was 4 times the(volume) of ASPs filtrate,the elimination rate of the production to ·OH could reach to the highest value of(68.2%.) The inhibition of ASPs to the lipid peroxidation of the erythrocyte membrane was improved with the increase of the concentration of ASPs.When the concentration of ASPs was above(0.4 g/L,) ASPs could not only inhibit the lipid peroxidation but also produce some reductive effect to the erythrocyte membrane.
【Fund】: 全国教育科学“十五”规划教育部重点项目基金(批准号:DIA030165)
【CateGory Index】: TQ464.1;
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