Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Jilin University(Science Edition)》 2012-02
Add to Favorite Get Latest Update

Cloning of GmNHX3 Gene from Glycine max L Merr and Construction of Its Genetic Transformation Vector

ZHANG Ji-xing1,2,WANG Xiao-yu3,CHEN Yong-sheng1,2,HUANG Feng-lan1,2,LI Guo-rui1,2(1.College of Life Sciences,Inner Mongolia University for the Nationalities,Tongliao 028000,Inner Mongolia AutonomousRegion,China;2.Engineering Research Center of Castor of Universities of Inner Mongolia Autonomous,Tongliao 028000,Inner Mongolia Autonomous Region,China;3.College of Plant Sciences,Jilin University,Changchun 130062,China)  
RACE(rapid-amplification of cDNAends) technique was used to clone Na+/H+ antiporter gene from Glycine max L Merr with the total RNA as the template.This gene was linked to the plasmid PBI121 and the expression vector PBI121-NHX3 was constructed.The results show its ORF(open reading frame)consists of 1 503 bp,which can be deduced to encode 501 amino acids.It was found that predicted amino acid sequence had an identification of 72%-94% compared with amino acid sequence from other ten plant species and it had a typical structural domain of the monovalent cation(proton) antiporter.The gene was named GmNHX3,(GenBank accession number: JN872904).Genetic transformation vector of PBI121-NHX3 was constructed successfully by identification method of PCR and restriction enzyme digestion.
【Fund】: 内蒙古自然科学基金(批准号:2010BS0502);; 内蒙古自治区高等学校研究项目(批准号:NJ10111)
【CateGory Index】: S565.1
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 5 Hits
1 Wang Quanwei 1 Li Xinling 1 Zhang Hailing 2 Xu Xiangling 1 1 Life Science and Technology Institute,Harbin Normal University,Harbin,150025;2 Institute of Pratacultural Sciences,Heilongjiang Academy of Agricultural Sciences,Harbin,150086;Overexpression of Na~+/H~+ Antiporter Gene(nhaA) Improves Salt Tolerance in Soybean[J];Molecular Plant Breeding;2010-04
2 Zhou G A, Guan R X, Li Y H, et al.;Molecular characterization of GmNHX2, a Na~+/H~+ antiporter gene homolog from soybean, and its heterologous expression to improve salt tolerance in Arabidopsis[J];Chinese Science Bulletin;2009-17
3 SONG Yazhen NAN Hongmei LIU Feng PAN Xinshe PEI Awei;Comparison Analysis of Homology,Identity and Similarity[J];China Terminology;2011-02
4 YU Han-song1,ZHANG Ji-xing2,LI Yan-fang3,MA Lan-qing4,HU Yao-hui1,(1.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China ;2.College of Life Sciences,Inner Mongolia University for the Nationalities,Tongliao 028043,China ;3.College of Plant Science,Jilin University,Changchun 130062,China ;4.Key Laboratory of Urban Agriculture(North),Ministry of Agriculture,Beijing University of Agriculture,Beijing 102206,China);Full-length cDNA Cloning of Glycosyltransferase Family from Rhodiola sachalinensis[J];Food Science;2010-21
5 Yao Yingqiu1 Chen Xing2 Wang Pu1 Liang Yan2 Yan Mei2 Xiao Bai2(1College of Life Science,Capital Normal University,Beijing 100037;2Beijing Chaoyang Hospital Affiliate of Capital of Medical Sciences,Beijing 100020);Improved Methods for Isolation of High Quality Total RNA from Glycine max Leaves[J];Biotechnology Bulletin;2008-01
Chinese Journal Full-text Database 4 Hits
1 LI Fei1,HE Yun-kun2,ZHANG Min1,LIU Jie3(1.Guizhou Potato Institute,Guiyang,Guizhou 550006;2.Guizhou Institute of Science and Technology Information,Guiyang,Guizhou 550004;3.Institute of Vegetable and Flower,China Academy of Agricultural Sciences,Beijing 100081,China);Extraction of Total RNA of Potato Seeding in Vitro and Its Quality Analysis[J];Guizhou Agricultural Sciences;2008-06
2 QIN Zheng-rui1,2,SUN Lei1,2,LI Hong-yu2(1.College of Plant Science,Jilin University,Changchun 130062,Jilin;2.Institute of Crop Sciences,Chinese Academy of Agricultural Sciences,Beijing 10081,China);Rapid Identification of GmCRY1 and GmCRY2 Function by Leaf Injection in Soybean[J];Soybean Science;2012-01
3 ZAHNG Ji-xing1,2,LI Guo-rui1,YU Han-song3(1.College of Life Sciences,Inner Mongolia University for Nationalities,Tongliao 028043,China;2.Key laboratory of urban agriculture(north)of ministry of agriculture of P.R.China,Beijing University of Agriculture,Beijing 102206,China;3.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118,China);Cloning of UGT3 Gene by Aspergillus niger Elicited from Rhodiola sachalinensis[J];Journal of Inner Mongolia University for Nationalities(Natural Sciences);2011-06
4 LIU Li-Ming1,2,3, LIU Hong-Yan1,2,3, and TIAN Bao-Ming1 1 Bioengineering Department, Zhengzhou University, Zhengzhou 450001, China; 2 Institute of Plant Protection, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China; 3 Henan Key Laboratory for Control of Crop Diseases and Insect Pests, Zhengzhou 450002, China;Selection of Reference Genes from Sesame Infected by Macrophomina phaseo-lina[J];Acta Agronomica Sinica;2012-03
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Fu Chang Wang Yu-Ying Dai Hong-Jie (Life and Environment College, Harbin Normal University, Harbin, 150080);ANALYSIS OF RNA LATION FROM DIFFERENT SOYBEAN SRGAS[J];Soybean Science;2004-04
2 Luo Zhenfeng Li Qiyun (Northeast Agricultural University, Haerbin 150030;Jilin Academy of Agricultural Sciences, Gongzhuling 136100);COMMERCIALIZATION STATUS AND RESEARCH PROGRESS OF TRANSGENIC SOYBEAN[J];Soybean Science;2005-03
3 WANG You hua 1,LU Meng zhu 2,DUAN Liu sheng 1 (1 College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China;2 Laboratory of Biotechnology,Institute of Chinese Forestry Academy,Beijing 100091,China);Modified Hot Phenol Method for Extracting Total RNA from Seedling Root of Cotton[J];Acta Botanica Boreali-occidentalia Sinica;2005-04
4 WANG Chun-li1,2,LIANG Zong-suo1(1 College of Life Science,Northwest A&F University,Yangling,Shaanxi 712100,China;2 Hybrid Rapeseed Research Center of Shaanxi Province,Dali,Shaanxi 715105,China);Research Progress of Signal Transduction Pathway and Regulation of Secondary Metabolism in Plant Induced by Exogenous Stimulus[J];Acta Botanica Boreali-Occidentalia Sinica;2009-05
5 Zhang Hongzhi, [Research Institute of Sports Team of People's Army of China,Beijing 100091〖HJ*8] WANG Chunquan,SUN Shuping,DONG Youchang.;Study on Effects of Rhodiola Like Medicines on Enhancing Sportsmen's Stamina.[J];SPACE MEDICINE & MEDICAL ENGINEERING;1996-03
6 YU Han-song1.2,ZHANG Ji-xing1,MA Lan-qing1,3,LI Yanfang1,HU Yao-hui2(1.College of Plant Science,Jilin University,Changchun 130062,China;2.College of Food Science and Engineering,Jilin Agricultural University,Changchun 130118, China;3.Institute of Botany,Chinese Academy of Sciences,Beijing 100093,China);Cloning of Glucosyltransferases by CODEHOP from Rhodiola sachalinensis[J];Journal of Jilin Agricultural University;2008-02
7 JIA Zhenping,et al(General Hospital of Lanzhou Command of PLA)WEI Hulai(Institute of Hematology,Lanzhou Medical Collage);Protective effects of R. Kirilowii on radiation[J];Journal of Lanzhou Medical College;1997-03
8 WANG Fang1,2, ZHANG Bin2, SHAN Lei2, BI Yu-ping2, XU Ze-yong3(1. School of Life Sciences, Shandong Normal University, Jinan 250014,China; 2. Plant Biology Technology Research Center, SAAS, Jinan 250100,China; 3. Oil Crops Research Institute ,CAAS,Wuhan 430062,China);An Improved Method for RNA Isolation from Peanut Seeds[J];Journal of Peanut Science;2002-04
9 MA Li,CAI Donglian,LI Huaixing,WANG Ying,PEI Suping,and SONG Lihua.Departmant of Nutrition,Changhai Hospital of the Second Military Medical University,Shanghai 200433,China;Antifatigue effects of salidroside on mice[J];Medical Journal of the Chinese People's Armed Police Forces;2007-11
10 WANG Li1,2,SHI Ling-Ling1,ZHANG Yan-Xia1,LIU Yu-Jun1(1.College of Biological Science and Biotechnology,Beijing Forestry University,Beijing 100083,China;2.Department of Medicine,Tibet Institute for Nationalities,Xianyang,Tibet 712082,China);Biosynthesis and Regulation of the Secondary Metabolites in Plants[J];Journal of Wuhan Botanical Research;2007-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved