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《Journal of Jilin University(Science Edition)》 2012-02
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Cloning of GmNHX3 Gene from Glycine max L Merr and Construction of Its Genetic Transformation Vector

ZHANG Ji-xing1,2,WANG Xiao-yu3,CHEN Yong-sheng1,2,HUANG Feng-lan1,2,LI Guo-rui1,2(1.College of Life Sciences,Inner Mongolia University for the Nationalities,Tongliao 028000,Inner Mongolia AutonomousRegion,China;2.Engineering Research Center of Castor of Universities of Inner Mongolia Autonomous,Tongliao 028000,Inner Mongolia Autonomous Region,China;3.College of Plant Sciences,Jilin University,Changchun 130062,China)  
RACE(rapid-amplification of cDNAends) technique was used to clone Na+/H+ antiporter gene from Glycine max L Merr with the total RNA as the template.This gene was linked to the plasmid PBI121 and the expression vector PBI121-NHX3 was constructed.The results show its ORF(open reading frame)consists of 1 503 bp,which can be deduced to encode 501 amino acids.It was found that predicted amino acid sequence had an identification of 72%-94% compared with amino acid sequence from other ten plant species and it had a typical structural domain of the monovalent cation(proton) antiporter.The gene was named GmNHX3,(GenBank accession number: JN872904).Genetic transformation vector of PBI121-NHX3 was constructed successfully by identification method of PCR and restriction enzyme digestion.
【Fund】: 内蒙古自然科学基金(批准号:2010BS0502);; 内蒙古自治区高等学校研究项目(批准号:NJ10111)
【CateGory Index】: S565.1
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