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《Jilin Animal Husbandry and Veterinary Medicine》 2008-04
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PCR Detection on Multidrug Resistance Gene AcrA of the E.coli

GUAN Li-zeng,JU Yu-lin (Agriculture College,YanBian University,Longjing Jilin 133400)  
The E.coli multidrug resistance strain separated from clinic is becoming more,and the E.coli multidrug resistance is mainly concerned with the AcrAB system that lies in the E.coli chromosome. In this experiment cyclopropane Sha Xing, tetracyclin and chloromycetin is firstly used to induce the sensitive E.coli to the multidrug resistance one.Then the gene gro-up DNA is extracted from the multiple drug resistance E.coli , using the AcrA gene code sequence that obtains from the gene bank design primer, and PCR is carried out on basis of the template of multidrug resistance E.coli gene group. The 1005bp piece is obtained through sequence analysis and compared with the sequence of the AcrA gene that lies in the gene bank , the homology is 99.8%. The result shows that :AcrA gene is concern with the E.coli. drug tolerance.
【CateGory Index】: S852.61
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