The effect of cyclooxygenase-2 and oxidative stress in acute hepatic injury
TIAN Geng, YU Jie ping, LUO He sheng, YU Bao ping, LI Jian ying (Department of Digestive Diseases, the People's Hospital of Wuhan University, Wuhan 430060,Hubei,China)
Objectives:To study the expression of cyclooxygenase 2(COX 2) in acute hepatic injury and the relationship between COX 2 and oxidative stress. Methods:Thirty Wistar rats were divided into three groups: normal group: 10 rats, control group: 10 rats ip injection of 1 ml/g of CCl 4, model groups: 10 rats ip injection of 1 ml/kg of CCl 4 and after two hours ig of 80 mg/kg of Celecoxib. Results:Levels of serum ALT,AST and LPS in control rats were higher than normal rats. The indices were different significantly between control rats and model rats( P 0.05). The levels of liver homogenate NO and MDA in control rats and model rats were higher than normal rats, the difference between control rats and model rats were significant statistically ( P 0.05). H E stain showed hepatic cell swelling, vacuolation and steatosis in control and model rats. Immunohistochemical stain showed that the expression of COX 2 in model rats were higher than control rats. Conclusions:After acute hepatic injury, endotoxin induced the expression of COX 2 oxidative stress, which enhanced hepatic injury. The increased expression of COX 2 and NO might act as a protective role.