Construction and expression of eukaryotic expression vector bearing fusion gene of human nm23-H_1gene and EGFP gene
CHE Guo-wei, ZHOU Qing-hua,LIU Lun-xu, QIN Yang, SUN Zhi-lin (The Key Laboratory of Lung Cancer Molecular Biology, West China Hospital, Sichuan University Chengdu 610041,Sichuan,China)
Objective:To construct and express a recombinant eukaryotic vector bearing fusion gene of human nm23-H_1gene and EGFP gene, and to explore tne mechanism of nm23-H_1 reversing the metastasis phenotype in lung cancer. Methods:pLXSN-nm23-H_ 1-EGFP is constructed by gene engineering and molecular clone technique .then the recombinant eukaryotic vector was transferred into PA317 cells by using Lipofectamine, and the virus of nm23-H_ 1-EGFP infected L9981 cells. Results:A retrovirus eukaryotic expression vector pLXSN-nm23-H_1-EGFP was successfully cells. Conclusion:The fusion protein of nm23-H_1-EGFP is stablely,continuously and high efficiently expressed in transfected L9981 cells.
【Key Words】：
nm23-H_1-EGFP fusion gene Gene expression L9981 Cell
【Fund】： 国家自然科学基金资助项目 (批准号 :3 0 43 0 3 0 0 ) ;; 中国博士后科学基金资助项目 (批准号 :2 0 0 40 3 5 697)
【CateGory Index】： Q782
【Fund】： 国家自然科学基金资助项目 (批准号 :3 0 43 0 3 0 0 ) ;; 中国博士后科学基金资助项目 (批准号 :2 0 0 40 3 5 697)
【CateGory Index】： Q782
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