Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Journal of Medical Postgraduates》 2005-07
Add to Favorite Get Latest Update

Analysis of promoter hypermethylation of p16 gene in sera from primary liver cancer patients

CHEN Long-bang~1, LIN Qing~1, TANG Yong-ming~2, WANG Jing~(2)(1. Department of Oncology, Nanjing General Hospital of Nanjing Military Command, PLA, Nanjing 210002,Jiangsu,China; 2.Laboratory of Clinical Immunology, the 454th Hospital of PLA, Nanjing 210002,Jiangsu,China)  
Objective:To analyze the aberrant methylation of p16 gene in sera from primary liver cancer patients and to evaluate the clinical significance. Methods:A methylation-specific PCR was performed for the detection of promoter hypermethylation of p16 gene in blood DNA from 64 cases of PLC patients,and to anamyze the relationship between the aberrant methylation of p16 gene and the clinical pathological data . Results:76.6%(49/64)of the sera from 64 cases of PLC patients showed hypermethylation for p16 promoter,whereas no methylated p16 gene promoter were found in sera from liver benign diseases patients and normal control. Methylated p16 gene promoter in sera did not strongly correlated with HBsAg, AFP,stage ,metastasis and differentiation in PLC. Conclusion:Detection of the aberrant methylation of p16 gene in blood DNA from PLC patients might offer an effective means for the earlier auxillary diagnosis of the malignancy.
【Fund】: 南京军区南京总医院科研基金重点资助项目(批准号:2003017)
【CateGory Index】: R735.7
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【Citations】
Chinese Journal Full-text Database 1 Hits
1 Zhou Qinghua, Shi Yingkang, Hou Mei, et al. Department of Thoracocardiac Surgery, The First University Hospital, West China University of Medical Sciences, Chengdu, Sichuan 610041, P.R. China;Study on the Mutation of p16 Gene in Human Non small Cell Lung Cancer[J];中国胸心血管外科临床杂志;1997-02
【Co-citations】
Chinese Journal Full-text Database 2 Hits
1 ZHU Weidong* ,WANG Xin* ,ZHANG Yibing. * Department of General Surgery,Tangshan People’s Hospital,Hebei, Tangshan 063001,China;Clinical significance of the expression of EZH2 and P16 protein in thyroid papillary carcinoma[J];河北医药;2010-18
2 SU Changqing *, YE Yukun, CAO Xiangrong, SHAN Xiangnian. * Molecular Biological Laboratory of Nanjing 81 Hospital, Nanjing 210002 P.R.China. E mail:sucq@jlonline.com;Homozygous deletion of CDKN2/p16 gene in lung cancers[J];中华医学遗传学杂志;1999-05
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved