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《Journal of Medical Postgraduates》 2005-10
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Intrapericardial injection with a trans-diaphragmatic approach in rats

HUI Hai-peng~1,LI Xiao-ying~1,PENG Jiang~2(1.Department of Geriatric Cardiology,General Hospital of PLA,Beijing 100853,China;2.Department of Orthopaedic Surgery,Wulumuqi General Hospital of Lanzhou Military Command,PLA,Wulumuqi 830000,Xinjiang,China)  
Objective:To study the feasibility,safety and effectiveness of intrapericardial injection with a trans-diaphragmatic approach in rats and its value in the study of heart diseases. Methods:Blue-black ink,recombinant adeno-associated virus carrying enhanced green fluorescent protein((eGFP)) gene,0.9% sodium chloride solution were respectively injected into pericardium of rats in three different groups(Group ink,Group eGFP and Group sodium chloride) by intrapericardial injection with a trans-diaphragmatic approach.Autopsy was done and hemodynamic parameters were measured and cryosection was analyzed by fluorescence microscopy. Results:The injection was proved successful in all rats in Group ink.Green fluorescence was detected in cryosection of all hearts in Group eGFP and the expression of green fluorescent protein was ubiquitously,but not homongeneous.No rat died during and after the operation among the operated rats(rats in Group eGFP and Group sodium chloride).There was no difference between hemodynamic parameters of the operated rats and those of the controls.Conclusion:The intrapericardial injection with a trans-diaphragmatic approach is a simple,feasible,safe and valid operation and suitable for small experimental animals.And it suggests an easy, safe,efficient and cheap technique in the study of heart diseases.
【Key Words】： Rat Injection Pericardial sac Operation
【Fund】： 国家自然科学基金资助项目(批准号:30470716);; 国家重点基础研究发展规划资助项目(973)(批准号:G2000056906)
【CateGory Index】： R541
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