Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Nonwood Forest Research》 2011-01
Add to Favorite Get Latest Update

Selection of internal control genes in semi-quantitative RT-PCR in Betula platyphylla

DAI Chao,LIU Xue-mei,ZHOU Fei(College of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China)  
In order to provide theoretical basis for researching lignin synthetic regulation,taking Betula platyphylla as investigated object,expression stability of four housekeeping genes in different tissues and periods were analyzed,which were 18S rRNA,Actin,Ubiquitin and Tubulin,respectively.The results showed that the expression of 18S rRNA,Actin and Ubiquitin were stable,and they could be used as internal control genes to analyze expression level of target genes in B.platyphylla.Tubulin was less stable,and could't be used as internal control genes to analyze expression of target genes.
【Fund】: 教育部科学技术研究重点项目(109053);; 中央高校基本科研业务费专项资金资助(DL09CA06)
【CateGory Index】: S792.153
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
【References】
Chinese Journal Full-text Database 8 Hits
1 SUN Feng-bin1,WEI Ji-cheng2,LIU Ying1,ZHANG Yan1,MA Li-li1,FU Yang-wei1,LIU Xue-mei1(1.College of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China; 2.Mudanjiang Normal University,Mudanjiang 157012,Heilongjiang,China);Isolation,transcription and expression of calmodulin gene CaM in Betula platyphylla[J];Nonwood Forest Research;2011-04
2 ZHANG Yan,LIU Ying,SUN Feng-bin,DAI Chao,LIU Xue-mei(College of Life Science,Northeast Forestry University,Harbin 150040,Heilongjiang,China);Isolation and Expression of APETALA2 Transcription Factor Gene in Betula platyphylla[J];Forest Research;2012-02
3 MA Li-Li QIU Li-Li FU Yang-Wei JING Rui-Rui LIU Xue-Mei(College of Life Science,Northeast Forestry University,Harbin 150040);Isolation and Expression of Flowering Locus T(FT) Gene in Betula platyphylla[J];Bulletin of Botanical Research;2012-06
4 XING Zhao-bin a,LONG Yue-hong a,LAO Feng-yun b,ZHUANG Peng-yu b,XIU Le-shan a(a.College of Life Science;b.College of Pharmacy,Hebei United University,Tangshan 063000,Hebei,China);Expression of squalene synthase gene in Eleutherococcus senticosus and its influence on saponins content[J];Nonwood Forest Research;2013-01
5 LI Chun-xue a,HUANG Ya-ting a,FAN Gui-zhi a,LIU Gui-feng b,WANG Xiao-dong a,LI Chun-xiao a,JI Xiao-ming a,SUN Mei-ling a,MA Ming-mei a,ZHAN Ya-guang a,b(a.College of Life Sciences;b.State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin 150040,Heilongjiang,China);Variation analysis on triterpenoid content in barks of trunk and branch in Betula platyphylla[J];Nonwood Forest Research;2013-01
6 GUAN Minxiao,LIU Xuemei,ZHANG Yan,LIU Ying,SUN Fengbin(College of Life Science,Northeastern Forest University,Harbin 150040,China);Isolation and transcription expression analysis of SPL8 transcription factors gene of Betula platyphylla[J];Journal of Nanjing Forestry University(Natural Sciences Edition);2013-03
7 XING Zhao-bin;MENG Chun-yan;XIU Le-shan;LAO Feng-yun;ZHUANG Peng-yu;College of Life Science,Hebei United University;College of Public Health,Hebei United University;College of Pharmacy,Hebei United University;;Expression of key enzyme genes in saponins biosynthesis and correlation between them and saponins content in different gender types of Eleutherococcus senticosus[J];Nonwood Forest Research;2013-03
8 Wang Bei;Guo Zhixiong;Zhong Fenglin;Pan Tengfei;Institute of Postharvest Science and Technology of Horticultural Products, Fujian Agriculture and Forestry University;College of Horticulture, Fujian Agriculture and Forestry University;;cDNA Cloning and Expression of β-tubulin in Plum (Prunus salicina var. cordata) Fruit[J];Chinese Agricultural Science Bulletin;2013-28
【Citations】
Chinese Journal Full-text Database 8 Hits
1 Yu Shujun; Wang Tian; Kim Jong Wook; Lee Jeong Sik. Journal of Beijing Forestry University (2001)23(1)24~28[Ch, 9 ref.] Department of Environmental Horticulture, The University of Seoul, Seoul 130-743, Korea.;Container culture experiments of Betula platyphylla (Ⅰ) Screening of the optimum formula for substrate.[J];Journal of Beijing Forestry University;2001-01
2 Song Funan,Xing Lei,Chen Su,Dai Chao,Liu Xuemei (Key Laboratory of Forest Tree Genetic Improvement and Biotechnology (Northeast Forestry University),Ministry of Education,Harbin 150040,P.R.China);Expression and Isolation of Phenylalanine Ammonia-lyase (PAL) Gene in Betula platyphylla[J];Journal of Northeast Forestry University;2009-12
3 LIU Xue-Mei1 YANG Chuan-Ping 2* GENG Feng3(1.College of Life Sciences, Northeast Forestry University, Harbin 150040) (2.Forestry College of Northeast Forestry University, Harbin 150040) (3.Beijing Normal University, Beijing 100083);Development of ovule and relation between embryo and endosperm developments of Betula platyphylla[J];Bulletin of Botanical Research;2005-03
4 Zhang Yumei,Yang Ling College of Chemical and Life Sciences,Zhejiang Normal University,Jinhua,Zhejiang 321004;Cloning and Analysis of Two Internal Control Gene Fragments from Phaseolus lunatus L.[J];Chinese Journal of Tropical Crops;2009-07
5 HE Li-hong1,2,ZHANG Jin-biao3*,BIN Jin-hua3(1.College of Life Science,Zhongkai University of Agriculture and Technology,Guangzhou 510225;2.College of Horticulture,South China Agricultural University,Guangzhou 510642;3.College of Life Science,South China Normal University,Guangzhou 510631);Research progress of phenylalanine ammonia-lyase[J];Food Science and Technology;2006-07
6 LI Yan1,2,LI Guan1(1.College of Life Science and Technology,Xinjiang Normal University,Urumqi 830046,China;2.South China Botanical Garden,Chinese Academy of Sciences,Guangzhou 510650,China);Cloning and Sequence Analysis of Actin Gene from Broussonetia papyrifera[J];Biotechnology;2007-04
7 Yu Pei-tao;Xue Ying-long Department of Biology,Fudan University;THE ROLE OF PHENYLANINE AMMONIA LYASE (PAL) IN CELL DIFFERENTIATION[J];;1987-01
8 ZENG Fan-Suo~1 NAN Nan~1 ZHAN Ya-Guang~(2,*) ~1College of Life Science,~2Key Laboratory of Forest Tree Improvement and Biotechnology of Ministry of Education,Northeast Forestry University,Harbin 150040,China;Extraction of Total RNA from Mature Leaves Rich in Polysaccharides and Secondary Metabolites of Betula platyphylla Suk.[J];Plant Physiology Communications;2007-05
【Co-citations】
Chinese Journal Full-text Database 10 Hits
1 YUAN Rui-ling et al(State Key Laboratory of Virology,College of Life Sciences,Wuhan University,Wuhan,Hubei 430072);Effect of HrpZ Excitation on Activities of Defense-related Enzymes in Several plants[J];Journal of Anhui Agricultural Sciences;2008-32
2 ZHAO Bai-hui et al (College of Biological Sciences and Biotechnology,Beijing Forestry University,Beijing 100083);Extraction Method of RNA from Pinus tabulaeformis Carr Mature Embryo[J];Journal of Anhui Agricultural Sciences;2010-06
3 WANG Kang-yu et al (College of Life Science,Jilin Agricultural University,Changchun,Jilin 130118);Effects of Elicitor Synergism on the Physiological and Biochemical Indices of Astragalus membranaceus(Fisch.)Bge. Calli[J];Journal of Anhui Agricultural Sciences;2010-14
4 PENG Xin et al(Zhejiang Pharmaceutical College,Ningbo,Zhejiang 315100);Changes of Phenolic Compounds Content and Activity of Enzymes Related to Phenolic Compounds in Fritillaria thunbergii Miq.Bulbs Stored at Different Cold Temperatures for Breaking Dormancy[J];Journal of Anhui Agricultural Sciences;2012-12
5 WEI Tao~1, CHEN Xi-yu~2, YANG Qiu-ting~1 ,LIU Chun-zhao~2(1.Beijing Laboratory of Bioactive Substances and Functional Foods of Beijing Union University, Beijing 100083,China; 2.State Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing 100080, China);Effect of Yeast Elicitors on Cell Growth and Suspension Cell Cultures[J];Journal of Beijing Union University;2005-03
6 Yu Shujun;Wang Tian;Kim Jong Wook; Lee Jeong Sik.Department of Environmental Horticulture,The University of Seoul,Seoul 130 743,Korea.;Container culture experiments of \%Betula platyphylla\% (Ⅱ) Selection of the optimum formula for substrate.[J];Journal of Beijing Forestry University;2001-02
7 CHEN Xin, WAN Deguang, YAN Zhu- yun, et al Chengdu University of Traditional Chinese Medidne(Chengdu, 610075, China);Gene Cloning of Phenylalanine Ammonia-lyase in Ginkgo Biloba[J];Journal of Chengdu Unversity of Tarditional Chinese Medicine;2004-01
8 ZHANG Yi,YAN Xiu-ming (College of Horticulture and Gardening,Yangtze University,Jingzhou,Hubei 434025,China);Changes of Pigments and Relative Substances in Leaves of Three Red-leafed Tree Species from Prune L. in Growing Seasons[J];Journal of Yangtze University(Natural Science Edition)Agricultural Science Volume;2008-04
9 HE Shengmi1, WU Aifang2, WANG Yafen3 ( 1.Vegetable Research Institute, Zhejiang Province Academy of Agricultural Sciences, Hangzhou 310021; 2.Shaoxing City Vegetable Technology Extension Station; 3.Shaoxing County Agriculture Technology Extension Center );Effect of Hot Pepper Stalk in Substrate on Hot Pepper Growth[J];Journal of Changjiang Vegetables;2010-16
10 LIU Mingzhi(Institute of Biotechnology,Changsha University,Changsha,Hunan 410003);Agrobacterium-mediated Transformation and the Characteristics of Sainfoin (Onobrychis Viciaefolia)[J];Journal of Changsha University;2005-05
China Proceedings of conference Full-text Database 3 Hits
1 JIA Shu-guo SHEN Yong-bao (College of Forest Resources and Environment,Nanjing Forestry University,Nanjing 210037);Characteristics of Embryo Germination of Sinojackia xylocarpa[A];[C];2007
2 LONG Hong-xu,TAN Xiao-feng,CHEN Hong,ZHANG Lin Hunan Changsha,The Key Lab of Non-wood Forest Products of State Forestry Administration,Central South University of Forestry and Technology,410004;Method for high-quality total RNA isolation from seeds of Vernicia fordii[A];[C];2009
3 Chen Ai-guo,Peng Dong,Chen Xiang-dong,Shi Yi,Liang H B (Tobacco researcher institute of CAAS /Key Laboratory of Tobacco Biology and Processing,Ministry of Agriculture, Qingdao Shandong266101);Studies on Relationships of Related Enzyme Activities and Polyphenol Products of Phenylpropanoid Metabolic Pathway in Flue-cured tobacco[A];[C];2012
【Co-references】
Chinese Journal Full-text Database 10 Hits
1 HUO Feng-mei1,2,MIAO Jun2*,ZHANG Yi-hui2,YANG Yan-yan2,LIU Bing-jiang2,HUO Yu-meng2,YANG Jian-ping1,WU Xiong2*(1.College of Horticulture Science and Engineering of Shandong Agricultural University,Taian 271018,China;2.Institute of Vegetables,Shandong Academy of Agricultural Sciences,Jinan 250100,China);Molecular Cloning and Expression Analysis of CHS Gene in Onion[J];Shandong Agricultural Sciences;2010-05
2 WANG Zhuo et al (Biological Science and Technology College,Shenyang Agricultural University,Shenyang,Liaoning 110161);Advances in Plant Actin[J];Journal of Anhui Agricultural Sciences;2007-10
3 JIN Ze-xin et al (Institute of Ecology,Taizhou College,Linhai,Zhejiang 317000);Analysis on the Secondary Metabolites in Different Nutrient Organs of Cyclocarya paliurus[J];Journal of Anhui Agricultural Sciences;2007-13
4 JIANG Zi-li(Biology Department of Zunyi Normal College,Zunyi,Guizhou 563002);DNA Methylation and Transgene Silencing in Plants[J];Journal of Anhui Agricultural Sciences;2009-12
5 SUN Tian-guo et al (School of Life Science and Engineering,Qiqihaer Unviersity,Qiqihaer,Heilongjiang 161006);Effects of Salt Stress on the Seed Germination of Melon and POD and SOD Activities in Its Seedlings[J];Journal of Anhui Agricultural Sciences;2009-24
6 LI Peng et al (School of Life Science,Southwest University,Chongqing 400715);Bioinformatic Analysis of Flavanone 3-hydroxylase in Plants[J];Journal of Anhui Agricultural Sciences;2010-06
7 GAN Hai-feng et al(Guangxi Citrus Research Institute,Guilin,Guangxi 541004);Effects of Drought Stress on Antioxidant System of the Leaves from Different Citrus Rootstocks[J];Journal of Anhui Agricultural Sciences;2010-35
8 SUN Yan 1,2 DAI Shao-jun 1 WEI Jian-hua 2 WANG Hong-zhi 2 *(1 Alkali Soil Natural Environmental Science Center,Northeast Forestry University,Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field,Ministry of Education,Harbin Heilongjiang 150040;2 Beijing Agricultural Biotechnology Research Center);Research Progress on Selection Marker Genes in Transgenic Plant[J];Modern Agricultural Science and Technology;2012-03
9 LI Zhi-liang1,2,3,XING Hao-chun1,YANG Qing2,WU Zhong-yi3,ZHANG Xiu-hai3,HUANG Cong-lin3(1.Department of Biology Science,Handan College,Handan,Hebei 056005,China;2.College of Life Science,Nanjing Agricultural University,Nanjing,Jiangsu,210095,China;3.Beijing Research Center of Agro-Biotechnology,Beijing 100097,China);Advancement of the Research on Transgenic White Clover[J];Northern Horticulture;2009-07
10 LIU Hai-yan1,QI Xiao-hui2,HAN Xue1 (1.Heilongjiang Forestry Vocational Technical College,Mudanjiang,Heilongjiang 157011;2.Horticulture College of Northeast Agricultural University,Harbin,Heilongjiang 150030);Effect of Drought and Water Recovers on Protective Enzymes Activities of 4 White Clovers[J];Northern Horticulture;2010-05
【Secondary References】
Chinese Journal Full-text Database 7 Hits
1 ZHENG Yi-ping;WEN Chao;ZENG Li-hui;College of Horticulture,Fujian Agriculture and Forestry Univertity/Institute of Genetics and Breeding in Horticultural Plants,Fujian Agriculture and Forestry University;;Cloning and sequence analysis of MYB gene from Narcissus pseudonarcissus[J];Subtropical Agriculture Research;2013-03
2 XING Zhao-bin a,LONG Yue-hong a,LAO Feng-yun b,ZHUANG Peng-yu b,XIU Le-shan a(a.College of Life Science;b.College of Pharmacy,Hebei United University,Tangshan 063000,Hebei,China);Expression of squalene synthase gene in Eleutherococcus senticosus and its influence on saponins content[J];Nonwood Forest Research;2013-01
3 CHEN Dong-liang1,PENG Zhen-hua1,2,GAO Zhi-min1(1.International Centre for Bamboo and Rattan,Key Laboratory on the Science and Technology of Bamboo and Rattan,Beijing 100102; 2.Research Institute of Forestry,Chinese Academy of Forestry,Beijing 100091,China);Cloning and Express Analysis of PeAP2 Gene and Its Promoter in Phyllostachys edulis[J];Forest Research;2013-02
4 GUAN Minxiao,LIU Xuemei,ZHANG Yan,LIU Ying,SUN Fengbin(College of Life Science,Northeastern Forest University,Harbin 150040,China);Isolation and transcription expression analysis of SPL8 transcription factors gene of Betula platyphylla[J];Journal of Nanjing Forestry University(Natural Sciences Edition);2013-03
5 XING Zhao-bin;MENG Chun-yan;XIU Le-shan;LAO Feng-yun;ZHUANG Peng-yu;College of Life Science,Hebei United University;College of Public Health,Hebei United University;College of Pharmacy,Hebei United University;;Expression of key enzyme genes in saponins biosynthesis and correlation between them and saponins content in different gender types of Eleutherococcus senticosus[J];Nonwood Forest Research;2013-03
6 HE Yansen1,2,YANG Biyun1,LI Ke1,CHEN Xiaojing1 1 College of Horticulture/Institute of Genetics & Breeding in Horticultural Plants, Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China 2 Mintai Horticulture Research Center,Fujian Academy of Agricultural Sciences,Zhangzhou,Fujian 363005,China;Changes of Flavonoids and Carotenoids Content in Narcissus Tazetta Flower[J];Chinese Journal of Tropical Crops;2013-03
7 YANG Ye;PAN Tengfei;PAN Dongming;Institute of Storage Science and Technology of Horticultural Products,Fujian Agriculture and Forestry University;;Cloning and Expression Analysis of NtMYB1 Gene from Narcissus tazetta var.chinensis[J];Chinese Journal of Tropical Crops;2013-08
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 Chen Youmin Wang Yuhua Yu Jiu(Landscape Architecture Department, BFU)Yu Shujun Zhang Zuoshuang Zhang Zhiming(Botanical Garden of Beijing Municipality) (Beijing Botanical Garden,Academia Sinica);A Study on the Introduction of Korean Azalea to Beijing Plain[J];Journal of Beijing Forestry University;1992-04
2 ZHAO Ying1,2,FU Hai-tian3,TIAN Wei-min1,JIANG Chang-shun1*(1.Key Laboratory for Tropical Crop Physiology,Ministry of Agriculture and Chinese Academy of Tropical Agricultural Science,Danzhou,Hainan Province 571737,China;2.College of Agronomy,Hainan University,Danzhou,Hainan Province 571737,China;3.Guangxi Subtropical Crops Research Institute,Nanning,Guangxi Province 530001,China);Changes in Defendant Enzyme Activity and Expression of PAL Gene of Stylosanthes after the Vaccination with Anthracnose[J];Acta Agrestia Sinica;2008-06
3 Xu Ning,Chen Xuefen,Chen Huacai,Chen Zongmao (Tea Research Institute,Chinese Academy of Agricultural Sciences,Hangzhou 310008);Morphological and Biochemical Parameters of Tea Varieties Resistante to Pink Mite(Acaphylla theae Watt)[J];JOURNAL OF TEA SCIENCE;1996-02
4 Wang Yucheng, Yang Chuanping, Jiang Jing(Northeast Forestry University, Harbin 150040, P. R. China);The Main Points and Principles of Isolating Total RNA from Ligneous Plant Tissues[J];Journal of Northeast Forestry University;2002-02
5 Yang Chuanping, Jiang Jing, Na Dongchen, Wei ZhigangNortheast Forestry University, Harbin 150040, P. R. China;Rapid Extracting RNA from Stamen Tissue of White Birch[J];Journal of Northeast Forestry University;2002-03
6 ZHAO De\|gang\ HAN Yu\|zhen\ GUO Feng\|li\ FU Yong\|fu\ MENG Fan\|jing (China Agriculturl University,College of Biological Sciences, Beijing 100094,China.);Studies on ultrastructure of stamens abortion during sex determination of ear in maize[J];JOURNAL OF CHINESE ELECTRON MICROSCOPY SOCIETY;1999-05
7 Ye Mingzhi~1 Ke Yuqin~1 Xia Yihou~2 Dai Liming~3 Wu Diming~1 (1 Dept. of Common Subjects; 2 Dept. of Plant Protection; 3 Dean's office, FAC, Fuzhou 350002);Changes of Several Isoenzymes in Rice Seedling After Bacterial Leaf Blight Infection[J];;1991-03
8 XU Li\|ping,CHEN Ru\|kai (Key Laboratory of Sugarcane Genetics and Breeding of Chinese Ministry of Agriculture, Fujian Agricultural University,Fuzhou 350002,China);Current status and prospects of smut and smut resistance breeding in sugarcane[J];FUJIAN JOURNAL OF AGRICULTURAL SCIENCES;2000-02
9 LIU Hai1,2,3,LIN De-qiu2, XU Jie3, JIANG Yue-ming1*(1South China Botanic Garden, Chinese Academy of Science, Guangzhou, Guangdong 510650 China; 2Biotech Research Institute, South China Normal University, Guangzhou, Guangdong 510631 China; 3Graduate School, Chinese Academy of Science, Beijing 100039 China);A Simple and rapid method for extracting RNA from banana fruit pulp rich in polysaccharides and polyphenolic compounds[J];Journal of Fruit Science;2006-01
10 Niu Zimian Wang Xianping Li Quan(Pomological Research Institute,Shanxi Academy of Agricultural Sciences,Taigu 030800)Bo Wansuo(Shanxi Academy of Agricultural Sciences)Li Runkai(Qingxu Agricultural Bureau);RELATIONSHIP BETWEEN ENDOGENOUS HORMONE CONTENT AND GROWTH AND FRUITING CHARACTERS OF APPLE FUJI STRAIN[J];HEBEI JOURNAL OF FORESTRY AND ORCHARD RESEARCH;1997-04
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved