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《Nonwood Forest Research》 2012-02
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Comparison of different extraction methods of total RNA from Ziziphus jujuba flower bud

FENG Yan-zhi,YUAN De-yi,ZHANG Lin,LONG Hong-xu,XU Jing-shi,LIU Min(The Key Lab.of Non-wood Forest Product of Forestry Ministry,Central South University of Forestry & Technology,Changsha 410004,Hunan,China)  
In order to study the optimal extraction method of total RNA from Ziziphus jujuba flower bud,using flower buds in Z.jujuba as materials,total RNA from Z.jujuba flower bud was extracted through improved CTAB method,Trizol method and 2 kinds of RNA extraction kit(Invitrogen PureLinkTM RNA Mini kit and RNA extraction kit from the Beijing Ao LaiBo Biological Technology Company).RNA concentration and electrophoregram were analyzed.The results proved that the method of RNA extraction kit from the Beijing Ao LaiBo Biological Technology Company could extract high-purity and highly-complete RNA,and it could be used in the molecular biology experiments,such as Northern hybridization,cDNA library establishment and semi-quantitative RT-PCR.
【Fund】: 国家林业公益性行业科研专项“南方鲜食枣规模化经营技术研究与示范”(201204407)
【CateGory Index】: S665.1
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Chinese Journal Full-text Database 8 Hits
1 LI Da-zhi1,2,DENG Zi-niu1,XIONG Xing-yao1,XIE Bing-yan 2 (1. College of Horticulture and Landscape,HNAU,Changsha 410128,China;2.Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences,Beijing 100081,China);Study on isolation of total RNA from tomato tissues[J];Journal of Hunan Agricultural University(Natural Sciences);2007-05
2 WEN Ya-feng,YUAN Qin-qin,HE Gang(Central South University of Forestry & Technology,Changsha 410004,Hunan,China);Advance in research on molecular breeding in Ziziphus jujuba Mill[J];Nonwood Forest Research;2010-02
3 LI Ji-dong1,WU Ying-xia1,FENG Jian-can1,BI Hui-tao1,LIANG Qi-wei1,KOU Shou-dao2(1.Henan Agricultural University,Zhengzhou 450002,Henan,China;2.Forestry Bureau of Xinzheng City,Xinzheng 451100,Henan,China);Compare of different extraction methods of RNA in Ziziphus jujuba[J];Nonwood Forest Research;2010-04
4 DONG Zhan-qiang,ZHAI Xiao-qiao,FAN Guo-qiang,ZHANG Zhi-kun(Institute of Paulownia,Henan Agricultural University,Zhengzhou 450002,China);Study on extraction methods of RNA from Paulownia leaves[J];Journal of Henan Agricultural University;2009-01
5 CHUN Jun,ZHENG Yan-Feng,WANG Sheng-Hua,CHEN Fang (College of Life Science,Sichuan University,Chengdu 610064,China);A RNA Isolation Method Suitable for a Wide Range of Materials[J];Progress in Biochemistry and Biophysics;2008-05
6 LIU Yi-ke~(1,2),SUN Hong-bo~2,JIAN Bo~2,HU Po~2,GAO Xiao-wei~2,HOU Wen-sheng~(1,2)(1 College of Agronomy,Northwest A & F University,Yangling,Shaanxi 712100,China;2 Crop Science Research Institute,Chinese Academy of Agricultural Sciences,Beijing 100081,China);Two efficient improved methods for isolation of high quality total RNA from soybean[J];Journal of Northwest Sci-Tech University of Agriculture and Forestry(Natural Science Edition);2006-12
7 WEN Ya-fenga,HE Gangb (a.School of Resources and Environment;b.School of Life Science and Technology,Central South University of Forestry and Technology Changsha 410004,Hunan,China);DNA Extraction Method and AFLP Reaction System of Ziziphus jujuba[J];Journal of Central South University of Forestry & Technology;2007-03
8 WEN Ya-feng1,HE Gang2,ZHANG Jiang3 (1.School of Resources and Environment,Central South University of Forestry and Technology,Changsha 410004,Hunan,China;2.School of Life Science and Technology,Central South University of Forestry and Technology,Changsha 410004,Hunan,China;3.School of Computer Science,Central South University of Forestry and Technology,Changsha 410004,Hunan,China);Research on a Molecular Identification System for Jujube Cultivars[J];Journal of Central South University of Forestry & Technology;2007-06
Chinese Journal Full-text Database 10 Hits
1 CHEN Lan-ying 1 ,ZHAO Zhen 1 ,WANG Xiao-tao 1 , QU Er-jun 1 ,CHEN Jian-nan 2,ˇ (1.Pingdingshan Technological Institute,Pingdingshan 467001,Henan,China;2.Chinese Academy of Sciences,Institute of Genetics and Developmental Biology,Beijing100101,China);High Level Expression of HSP70 Gene in Tissues of Gastric,Esophageal and Cervical Carcinomas and Its Relationship to Carcinogenesis[J];Carcinogenesis,Teratogenesis and Mutagenesis;2005-04
2 CHENG Feng1,2,SHENG Yan2,SHI Jing-yi2, CHEN yi2, XU Chao2, LIU Zhi2,LIANG Jun2, ZHU Zhong-yong1, SONG Huai-dong2 (1.PLA Center for Laboratory Medicine, Fuzhou General Hospital,Nanjin Military Command, Fuzhou 350025,Fujian China; 2. State Key Lab for Medical Genomics,Shanghai Institute of Hematology, Ruijin Hospital, Shanghai Jiaotong University, Shanghai 200025);Overlap-extension PCR Based Site-directed Mutants of UGRP1 Gene Promoter[J];Carcinogenesis,Teratogenesis & Mutagenesis;2007-01
3 WEN Ju-yi1,ZHANG Ji-ren2,LI Peng2,ZHANG Jun1,WANG Bin1(1.Department of Radiotherapy and Oncology,The Navy General Hospital,Beijing 100037;2.Center of Oncology,Zhujiang Hospital of Southern Medical University,Guangzhou 510280,China);Effect of NADH on Mutations of p53 Gene and Expression of c-erbB2 Gene in L02 Cells Induced by Diethylnitrosamine[J];Carcinogenesis,Teratogenesis & Mutagenesis;2009-05
4 JIN Yi-feng1,LI Tie-chen2,WANG Ying1,*,LIU Dong-hua11,SUN Zhen-gui1,(1. Department of Respiratory Medicine,the Affiliated Yijishan Hospital of Wannan Medical College,Wuhu 241001;2.Laboratory of Molecular Biology,Wannan Medical College,Wuhu 241001,Anhui,China);Single nucleotide polymorphisms in ERCC1 and XPD genes and sensitivity to platinum-based chemotherapy in non-small-cell lung cancer[J];Carcinogenesis,Teratogenesis & Mutagenesis;2010-05
5 WANG Ji-ying,WU Ying~*,GUO Jian-feng,HU Hong-mei,ZHANG Yin(The Veterinarian and Animal Husbandry Institute,Shandong Academy of Agricultural Sciences;The Key Lab of Disease Control and Breeding of Animal of Shandong Province,Jinan 250100,China);Frequency Changes of FSHβ and ESR Gene and Genotype in the Preserved Population of Laiwu Pigs[J];Shandong Agricultural Sciences;2007-01
6 CHEN Jing,HU Xiao-hui,MIAO Hua-rong,ZHANG Jian-cheng,YU Shan-lin(Shandong Peanut Research Institute,Qingdao 266100,China);Optimization of SRAP-PCR Reaction System on Peanut[J];Shandong Agricultural Sciences;2008-05
7 CHEN Chang-bao,ZHU Shu-hua,ZHOU Jie(College of Chemistry and Material Science,Shandong Agricultural University,Taian 271018,China);Improved CTAB Method for Total RNA Extraction of Mature Feicheng Peach Fruit[J];Shandong Agricultural Sciences;2009-05
8 GE Hong-hua~1,XIAO Ya-zhong~2, YAO Jian-ping~3(1. Modern Experiment Center, Anhui University,Hefei230039;2. School of Life Sciences, Anhui University, Hefei230039;3. Anhui Institute of Biology,Hefei230031,China);Construction of pET22b human ventricular myosin light chain 1 and its expression in E. coli BL21[J];Journal of Anhui University(Natural Sciences);2004-06
9 Hou Xindong~1 Sheng Guilian~1 Zhang Hua~2 et al(1 College of Environment,China University of Geosciences,Wuhan 430074;2 Agronomy College,Xinjiang Agricultural University,Wulumuqi 830052);A Research for Methods of Extracting Plasmid DNA[J];Anhui Agricultural Science Bulletin;2006-13
10 Dai Yongxin Yang Xiangkun Wang Lin(Department of Horticulture,College of Agriculture,Shihezi University,Xinjiang 832003);Methods for Rapidly Extracting Total RNA from Stone Fruits[J];Anhui Agricultural Science Bulletin;2007-07
China Proceedings of conference Full-text Database 10 Hits
1 Hai long Zhang~1 Zhao feng Tian~2 Zhi Dong~1 Yan ru Zhang~3 Fei yun Zhang~1 (1 College of Life sciences,1 Capital Normal University,Beijing 100048) (2 Institute of Plant and Environment Protection,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097) (3 Beijing City University,Beijing 100083);Efficient Expression of RMotV Coat Protein gene in E.coli[A];[C];2010
2 LI Mei,LIAO Baowen (The Research Institute of Tropical Forestry,CAF,Guangzhou 510520,China);Isolation and Screening of Hydrocarbon-degrading Bacterial Strains from the Mangrove Wetland Soils[A];[C];2011
3 GONG Shu-fang YANG Tao CHE Dai-di (Northeast Agricultural University.Harbin 150030);Observing the Activity of the Protein from the Rhizome Elytrigia repens to Modify the Ice Crystal[A];[C];2007
4 Wang Ying~1 Teng Qihui~1 Cao Hui~1 Hu Feng~2 Cui Zhongli~1 1 Key Laboratory of Microbiological Engineering of Agricultural Environment, MOA, Nanjing Agricultural University, Nanjing 210095 2 College of Resources and Environmental Sciences, Nanjing Agricultural University, Nanjing 210095;THE STUDY OF BACTERIA DIVERSITY AND SPACE DISTRIBUTION IN DIFFERENT CULTIVATION CONDITIONS PADDY FIDLDS[A];[C];2007
5 Cui Bao-an Wei Zhan-yong (The College of Animal Husbandry And Veterinary,Henan Agricultural University,Zhengzhou Henan 450002);Molecular Cloning and Antigenicity Analysis the VP2 Gene of PPV Vaccine Strain[A];[C];2008
6 Hu Gong-zheng Sun Ya-wei Chen Hong-ying Si Hong-bin Yuan Li Deng Li-xing Mo Juang Li Sheng-li (Collage of Animal Husbandary and Veterinary Medicine Engeming,Henan Agricultural University,Zhengzhou 450002,China);Genotypes of the extend spectrumβ-lactamase(ESBLs) produced by the shigella isolated from the fowl[A];[C];2008
7 ZhAO-QIBO Gao-Yan Jiang-Cong Jun (Economic crops in Xinjiang Academy of Agricultural Sciences Research Institute,Nanchang Road,Urumqi,Xinjiang 83000,China);The AFLP Analysis of G.barbadense HPFW356 with Xinhai NO 16[A];[C];2009
8 CUI Xiao -yan, FANG Ding - zhi, and LIU Bing - wen. Department of Biochemistry and Molecular Biology, West China School of Preclinical and Forensic Medicine, Sichuan University, Chengdu 610041, China;Screening of prothrombotic state-related genes from human hepatic cDNA library[A];[C];2004
9 WANG Zhi-yu,WANG Jian-wei,HE Shen-yi,WU Wei-ping,HAN Jin-xiang, HONG tao (Shandong Provincial Institute of Virology/Shandong Provincial Key Laboratory for Medical Virology and Key Laboratory of Biotechnological Pharmaceuticals, Ministry of Health, Jinan 250012, China;National Institute for Viral Disease Control and Prevention, China CDC, Beijing 100052, China;Institute of Pathogenic Biology, Medical School of Shandong University, Jinan 250012, China);Efficient expression of truncated Group A rotavirus VP6 in E.coli[A];[C];2006
10 Zhang Juan~1 Zhao Li~2 Wei Li~1 Liu Jiantao~2 Zeng Zhijiang~(1*) 1 Honeybee institute of Jiangxi Agriculture University,Nanchang 330045,China; 2 School of Life Science,Jiangxi Normal University of Science and Technology,Nanchang 330013,China;Effect of storage temperature and time on water-soluble proteins of royal jelly[A];[C];2007
【Secondary Citations】
Chinese Journal Full-text Database 10 Hits
1 WANG Jia et al(College of Landscape Architecture,Beijing Forestry University,Beijing 100083);Study on Optimization for ISSR Reaction System of Peony with Orthogonal Design[J];Journal of Anhui Agricultural Sciences;2006-24
2 Chen Xing Wang Qi Huang Yingchun Wang Xinsheng Wang Yingdian (College of Life Sciences, Beijing Normal University, 100875, Beijing, China);METHOD FOR RNA ISOLATION FROM RICE CARYOPSES[J];Journal of Beijing Normal University(Natural Science);2005-01
3 DUYingying,LIANG Yuerong,ZHANG Guanghui,LU Jiangliang(Zhejiang University Tea Research Institute, Hangzhou 310029);Extraction of total RNA from white tea and non-green tissues in Camellia sinensis[J];Journal of Tea;2007-01
4 PENG Jian ying 1, SHU Huai rui 2, PENG Shi qi 1 (1 Research Center of Chinese Jujube, Agricultural University of Hebei, Baoding 071001, China; 2 Department of Horticulture, Shandong Agricultural University, Tai′an 271018, China);A method for genomic DNA isolation of Chinese jujube and acid jujube[J];Journal of Agricultural University of Hebei;2000-04
5 LI Xue-ying,PENG Jian-ying,PENG Shi-qi(College of Horticulture,Agricultural University of Hebei,Baoding 071001,China);Comparison between extracting methods for genomic DNA of dry leaf of Ziziphus Mill.saved in silica[J];Journal of Agricultural University of Hebei;2006-01
6 JIANG Chang jun,WANG Zhao xia,LI Ye yun [WT6BX](Key Laboratory of Tea Biotechnology,Ministry of Agriculture,Anhui Agricultural University, Hefei 230036 )[WT5”HZ];Studies on Isolation of General RNA from Tea Plant [Camellia sinensis(L )O Kuntze][J];JOURNAL OF TEA SCIENCE;2000-01
7 LI Jing peng ,HE Cheng qang, WU Dan,REN Gui ping, LING Hua,LU Yun zhe (NEAU Agricultural University,Harbin 150030,PRC);PURIFYING mRNA BY ONE STEP[J];JOURNAL OF NORTHEAST AGRICULTURAL UNIVERSITY;1999-03
8 Fu Chang Wang Yu-Ying Dai Hong-Jie (Life and Environment College, Harbin Normal University, Harbin, 150080);ANALYSIS OF RNA LATION FROM DIFFERENT SOYBEAN SRGAS[J];Soybean Science;2004-04
9 纪徐淮,潘卫,崔晓红,戴晓晶,李石;Observation of chromosome numbers in 16 species of medicinal plants[J];ACADEMIC JOURNAL OF SECOND MILITARY MEDICAL UNIVERSITY;1995-02
10 WANG You hua 1,LU Meng zhu 2,DUAN Liu sheng 1 (1 College of Agronomy and Biotechnology,China Agricultural University,Beijing 100094,China;2 Laboratory of Biotechnology,Institute of Chinese Forestry Academy,Beijing 100091,China);Modified Hot Phenol Method for Extracting Total RNA from Seedling Root of Cotton[J];Acta Botanica Boreali-occidentalia Sinica;2005-04
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