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《Nonwood Forest Research》 2012-04
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Establishment of regeneration system for mature embryo in Vernicia fordii

LI Ze,TAN Xiao-feng,ZHANG Lin,LONG Hong-xu,YUAN Jun,FAN Xiao-ming,ZENG Yan-ling(Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees,Ministry of Education,Central South University of Forestry & Technology,Changsha 410004,Hunan,China)  
In order to establish quickly and efficiently regeneration system in Vernicia fordii,using embryos of "Putaotong" as materials,effects of storage method,sterilizing time,culture mediums and culture conditions on regeneration system in V.fordii were studied.The results show that no significant difference is presented between sand storage and natural conditions on establishment of regeneration system for embryo in V.fordii.The optimal disinfection time of embryos is 3 minutes by 0.1% mercuric chloride.The appropriate medium of embryo is 1/2MS supplemented with 0.5 mg/L IAA,and seedling rate is up to 98%.A large number of calli are generated from embryo by adding 6-BA in the culture medium,which is not suitable for regeneration system establishment in V.fordii.The optimum pH value of culture medium is 5.5.Compared with shading conditions,lighting can shorten the period of establishing regeneration system of embryo.
【Fund】: 国家林业公益性行业科研专项(201204403 200904023);; 湖南省自然科学基金项目(10JJ4022)
【CateGory Index】: S794.3
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