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《Acta Anatomica Sinica》 1989-01
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GLIA MATURATION FACTOR SUPPORTS THE SURVIVAL OF CEREBELLAR CORTICAL NEURONS IN PRIMARY CULTURE

Guo Wanhua Ramon Lim~* (Department of Histology and Embryology,Sun Yat-Sen University of Medical Sciences,Guangzhou.~*Department of Neurology,Division of Neurochemistry and Neurobiology,University of Iowa College of Medicine,Iowa City,USA)  
Glia maturation factor(GMF)detected in the adult brain is an acidic protein that has the ability to promote the morphological and chemical differentiation of astroblasts,but this effect is reversible.In the present study we have established neuron-enriched dissociated primary cultures from 7-day-old rat cerebellar cortex in which about 97% of the cells are neurons especially granule cells using differential count of indirect immunofluorescence.The addition of purified GMF to the cultures markedly enhances neuronal survival,while control cultures grown in the absence of GMF exhibit a significant decrease in neuronal number.These GMF effects are dose-dependent,with optimal stimulation occurring at a concentration of 250 ng/ml. Although the mechanism by which purified GMF influences cerebellar cortical neuronal survival is not known,these results suggest that GMF not only affects glial cells,but also may function as a neurotrophic agent in the central nervous metsys
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