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《Acta Anatomica Sinica》 1989-03
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LOWER DOSE HELIUM-NEON LASER IRRADIATION PROMOTING NEURONAL GROWTH AND RNA SYNTHESIS IN CULTURE

Zhou Hancheng Guo Wanhua Zeng Yuanshan Chen Qihui (Department of Histology and Embryology,Sun Yat-Sen University of Medical Sciences,Guangzhou)  
The superior cervical ganglia (SCG) were dissected from neonatal rats.Dissoci- ated cell cultures were grown in Eagle's MEM supplemented with 20% calf serum which contain nerve growth factor (NGF). The cultures were divided into two groups:laser irradiation group and control. The former was exposed to lower dose of Helium-Neon laser (power:4 milliwatt) 5 minutes every two hours.Two groups cultured for 20,22,24 and 28 hours respectively.Then the length of neurites of neurons were measured.At 22 hours, RNA and DNA synthesis of neurons labeled by ~3H-uridine and ~3H-thymidine were observed. The results showed that lower dose of Helium-Neon laser irradiation not only promotes growth of neurite but also enhances RNA synthesis of neurons in culture. However DNA synthesis in neurons does not promote in this experiment.
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