THE GROWTH AND PROLIFERATION OF HUMAN UMBILICAL ARTERY ENDOTHELIAL CELLS IN PRIMARY CULTURE
Wang Ruimian, Yan Sixin Meng Yang ,Rong Chengxing(Department of Histology and Embryology , Department of Oral Anatomy and Physiology , Hubei Medical UniversityDepartment of Histology and Embryology.Hubei Medical University, Wuhan 430071 ,China
The human umbilical artery endothelial cells (HUAECs) were successfully cultured without using attachment agents or growth factors. HUAECs were seeded at a density of 1 × 105/cm2. The morphology,growth and proliferation of HUAECs were studied with HE staining,and their ultra-structure by transmission electron microscopy (TEM). (1) By 24h after seeding ,the HUAECs grow and form many islet-like cell clusters. By 48h,the cell clusters increase in size and can be divided into a central region with crowded endothelial cells and a peripheral region with fewer cells. By 72h,cell clusters coalesce to form incomplete monolayer. By 216h,the senescent cells shed. (2) HUAECs are relatively elongated polygonal in shape and connect with neighboring cells by short processes. The endo-plasm of HUAEC contains weak basophilic granules,while the ectoplasm is flat and thin. (3) The average cell number in a cell cluster is 17. 39±3. 79 by 24h,131. 14±22. 83 by 48h,454. 16±111. 39 by 72h respectively. These results indicate that the cell population doubled 9 times within 72h. (4) In exponential proliferating 24-72h,many amitotic figures appear but the mitotic figure is not observed. By 120h and 216h when cell growth slows down the mitotic figures appear. (5) The Weibel-Palade bodies in HUAEC are observed under TEM.
【CateGory Index】： R329