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《ACTA ANATOMICA SINICA》 2000-04
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CELL PROLIFERATION, APOPTOSIS AND THE EXPRESSION OF RELATED GENES IN THE DEVELOPING LUNG OF MOUSE

WANG Qiang, HUANG Zhong\|xin *, XIA Chao\|yong (Department of Histology and Embryology, Medical College Jinan University, Guangzhou\ 510632, China)  
Objective\ This study is to explore the dynamic trends of cell proliferation, apoptosis and the expression significance of involved genes as p53 and bcl\|2 in developing lungs of mouse. Methods\ Lung tissues were taken from different developmental phases of Kunming mouse. Immunohistochemistry and TUNEL techniques were adopted to observe the variation of cell proliferation and apoptosis and the expression characteristics of p53 and bcl\|2. Results\ 1 From the 14th to the 16th day of gestation the positive ratio of PCNA reached its peak. After that, it descended gradually. From the 4th day to the 14th day after birth, the proliferation maintained in low level; 2 Two peaks of apoptosis emerged during the development of lungs. The first one appeared from the 14th to the 16th day of gestation; the second one appeared at the 7th day after birth; 3 The expression of bcl\|2 first appeared from the 19th day of gestation to the 14th day after birth, with constant expression sites in the epithelial cells lined in the proximal bronchia and the smooth muscle cells. 4 The expression of p53 was never seen in all phases during the developmental process. Conclusion\ 1 During the development of mouse lungs, cell proliferation was accompanied by apoptosis. The dynamic changes of both ratios might be correlated to the different structures appearing in different developmental phases; 2 The possible function of bcl\|2 during the lung developmental process might be that it prevented the main frame structures of the bronchial tree and the capillary network from being destroyed by apoptosis; 3 Apoptosis during the development of lung might be independent of the regulation of p53.\;
【Fund】: 国家自然科学基金! (39770 30 0 )
【CateGory Index】: R730.3
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