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《Acta Anatomica Sinica》 2001-04
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CHEN Xue yan, CHEN Shi ping, JIN Shu min,CHENG Kai, DONG Hong yan, CHEN Ke quan  (Department of Cell Biology, Peking Union Medical College, Beijing\ 100005,China)  
Objective To determine the cellular localization and expression pattern of AR and FSHR in adult rat testis must be helpful to understand the action site and mechanisms that T and FSH regulate spermatogenesis. Methods We applied in situ Hybridization to detect the expression of AR and FSHR on adult testis, in which Dig labeled cRNA probe was used to carry out the experiment on frozen sections; at the same time, following the technique of transillumination assisted microdissection we separated seminiferous epithelium into four stages(Ⅱ Ⅵ,Ⅶ Ⅷ,Ⅸ Ⅻ and ⅩⅢ Ⅰ), extracted total RNA and carried out dot hybridization, using α 32 P labeled cDNA probe, in order to test qualitatively and quantitatively the location of AR and FSHR mRNA and their expression pattern in adult rat testis. Results Our results showed that the positive signal of AR mRNA was located in Sertoli cells and Leydig cells. The signal in Sertoli cells began to appear in Ⅱ Ⅵ stages, strongest in Ⅶ Ⅷ stages and weakest in Ⅸ Ⅰ stages ( P 0 01). The positive signal of FSHR mRNA was located in Sertoli cells strongest in ⅩⅢ Ⅰ stages, weakest in Ⅶ Ⅷ and intermediate in the other stages ( P 0 01). The different pattern of stage specific expression of AR and FSHR in adult rat testis suggested that T and FSH act on different stages during spermatogenesis. Conclusion We suppose that there is a coaction at regulation spermatogenesis between T and FSH. From this point of view, it may provide new ideas for designing contraceptive strategies and treating human infertility.
【Fund】: 国家“九五”攀登计划预选项目资助课题 (95 预 3 3 )
【CateGory Index】: R321
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