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《Acta Anatomica Sinica》 2006-05
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BCL-2 METHYLATION IN THE DEVELOPMENT OF BREAST CANCER AND THE RELATIONSHIP BETWEEN BCL-2 METHYLATION AND EXPRESSION

LI Hong-zhi 1*,WANG Jia-zhen2, PENG Ying1, LI Dong1(1.Department of Biology;2. Medical health,Wenzhou Medical College, Wenzhou 325035,China)  
Objective To establish MSP (methylation-specific PCR) method for detecting bcl-2 gene, to study bcl-2 methylation in the development of breast cancer and the relationship between bcl-2 methylation and expression. Methods Designing the MSP primer of bcl-2 gene, the methylations in CpG island of bcl-2 gene 5'-regulatory region in 30 cases of normal, 25 cases of atypical hyperplasia(precancerous condition), 40 cases of lymphnode(-)(low-stage) and 45 cases of lymphnode(+)(high-stage) breast tissue were detected by using MSP. The expressions of bcl-2 were detected by using SP immunohistochemical technique. Results The positive rates of bcl-2 methylation in normal, atypical hyperplasia, lymphnode(-) and lymphnode(+) breast tissue were 10.0%,32.0%,40.0% and 53.3% respectively. The methylation of bcl-2 was increased and the expression of bcl-2 was decreased in the development of breast cancer(P0.01). The positive rate of bcl-2 methylation in atypical hyperplasia was significantly higher than that in norrnal(P0.05). There was always a significant negative correlation between the bcl-2 methylation and the bcl-2 expression in the above four developing stages of breast cancer(P0.01). Conclusion This study established MSP method for detecting bcl-2 gene. The design of the MSP primer of bcl-2 gene in this study was successful. The positive rate of methylation of bcl-2 promoter would become a molecular indicator of breast carcinogenesis. In the development of breast cancer, the methylation in CpG island of bcl-2 gene 5'-regulatory region could be a down-regulation factor of the expression.
【Fund】: 浙江省教委基金资助项目(981155);; 温州市科技局基金
【CateGory Index】: R737.9
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