Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Anatomica Sinica》 2006-05
Add to Favorite Get Latest Update


LI Hong-zhi 1*,WANG Jia-zhen2, PENG Ying1, LI Dong1(1.Department of Biology;2. Medical health,Wenzhou Medical College, Wenzhou 325035,China)  
Objective To establish MSP (methylation-specific PCR) method for detecting bcl-2 gene, to study bcl-2 methylation in the development of breast cancer and the relationship between bcl-2 methylation and expression. Methods Designing the MSP primer of bcl-2 gene, the methylations in CpG island of bcl-2 gene 5'-regulatory region in 30 cases of normal, 25 cases of atypical hyperplasia(precancerous condition), 40 cases of lymphnode(-)(low-stage) and 45 cases of lymphnode(+)(high-stage) breast tissue were detected by using MSP. The expressions of bcl-2 were detected by using SP immunohistochemical technique. Results The positive rates of bcl-2 methylation in normal, atypical hyperplasia, lymphnode(-) and lymphnode(+) breast tissue were 10.0%,32.0%,40.0% and 53.3% respectively. The methylation of bcl-2 was increased and the expression of bcl-2 was decreased in the development of breast cancer(P0.01). The positive rate of bcl-2 methylation in atypical hyperplasia was significantly higher than that in norrnal(P0.05). There was always a significant negative correlation between the bcl-2 methylation and the bcl-2 expression in the above four developing stages of breast cancer(P0.01). Conclusion This study established MSP method for detecting bcl-2 gene. The design of the MSP primer of bcl-2 gene in this study was successful. The positive rate of methylation of bcl-2 promoter would become a molecular indicator of breast carcinogenesis. In the development of breast cancer, the methylation in CpG island of bcl-2 gene 5'-regulatory region could be a down-regulation factor of the expression.
【Fund】: 浙江省教委基金资助项目(981155);; 温州市科技局基金
【CateGory Index】: R737.9
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
Chinese Journal Full-text Database 1 Hits
1 Min Daliu, Wang Baotai, Meng Gang et al (Dept of Pathol, Anhui Medical University, Hefei 230032);Apoptosis and immunohistochemical bcl 2、PCNA expression in breast carcinoma and precancerous lesions[J];CHINESE JOURNAL OF CLINICAL AND EXPERIMENTAL PATHOLOG;1999-02
Chinese Journal Full-text Database 10 Hits
1 LI Hong zhi, WANG Zong min, JIN Long jin, et al. (Department of Biology, Wenzhou Medical College, Wenzhou\ 325027, China);THE RELATIONSHIP BETWEEN THE GENE MUTATION,ABNORMAL EXPRESSION AND THE OCCURANCE, DEVELOPMENT, PROGNOSIS OF BREAST CANCER[J];Carcinogenesis,Teratogenesis and Mutagenesis;2002-04
2 HAN Jing-Qi,WANG Feng-Mei,YE Jun-LingDepartment of Pathology,Affiliated Hospital of Qinghai Univer-sity,Xining 810001,China;Expressions of ER,PR,P53,C-erbB-2 and PCNA in breast cancer tissues and their clinical significance[J];Journal of the Fourth Military Medical University;2008-07
3 Tang Hongwu 1* , Chen Guanquan 1, Zeng Yun’e 2 (1. Center of Instrumental Analysis, 2. Department of Chemistry, Wuhan University, Wuhan, 430072);Instrumental Analytical Methods in Modern Cell Analysis[J];Journal of Analytical Science;1999-06
4 BIN Xiaonong1 TAN Min2 HE Qinglian2 ZHAO Hong21Guangzhou medical college, Guangzhou, 510182, China2The Second Affiliated Hospital of Guangzhou University of TCM, Guangzhou 510120;Study of Apoptosis and Proliferation in breast tissue Carcinogenesis[J];International Medicine & Health Guidance News;2006-12
5 XIAO Xiu-li,Wei Bing,LONG Han-an,et al.(Department of Pathology,West China Hospital,Sichuan University,Chengdu,610041 China);The Study of the Relationships between Cell Proliferation and Apoptosis andER?PR?C-erbB-2 Expression in Breast Duct Epithelium Lesions[J];West China Medical Journal;2005-01
6 JIN Guo\|ying(Hangzhou Education College, Hangzhou 310020,China);A research for apoptosis[J];JOURNAL OF ZHEJIANG UNIVERSITY(SCIENCES EDITION);2000-01
7 LI Yun qing , ZHANG Si zhong, XIAO Cui ying (Department of Medical Genetics, West China Hospital, Sichuan University , Chengdu 610041,China);The complex methylation patterns of BRCA1 promoter[J];Basic Medical Sciences and Clinics;2001-04
8 He Jianmiao,Pu Yongdong,Ma Xiaojun,et al.Department of General Surgery,Second Affiliated Hospital,General Hospital of PLA,Beijing 100091,China;Apoptosis and apoptosis-regulating genes in advanced breast cancer after preoperative regional intra-arterial chemotherapy[J];Medical Journal of Chinese People's Liberation Army;2007-02
9 Xiao Ningxin 1, Li Zhonghong 2, Huang Huazhen 2, Jiang Zhijian 3 ( 1Department of Pathology and 2Department of Surgery, Jiangmen City Peoples Hospital,Jiangmen Guangdong 529000);Correlation of p~(21)~(WAF1) and PCNA expression to survival of the patients with breast carcinomas[J];Chinese Journal of Clinical and Experimental Patholog;2001-01
10 Zhang Jingli, Zhang Hongying, Bu Hong, Zhang Jie, Yu Ping, Wei Bing, Zhang Zhang (Department of Pathology, West China Hospital of Sichuan University, Chengdu 610041);Comparison between expression of Ki-67 and PCNA in breast carcinoma[J];Chinese Journal of Clinical and Experimental Pathology;2003-01
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved