Full-Text Search:
Home|Journal Papers|About CNKI|User Service|FAQ|Contact Us|中文
《Acta Anatomica Sinica》 2008-01
Add to Favorite Get Latest Update

AN COMPUTERIZED 3D RECONSTRUCTION METHOD BASED ON RAT'S CERVICAL CORD SERIAL SECTIONS

WU Hui-qun~1,L Guang-ming~(1),TANG Le-min~(2),JI Da-feng~1,GU Xiao-song~3(1.Department of Anatomy,Institute of Neurobiology,Key Laboratory of Neural Regeneration of Jiangsu Province,Medical School of Nantong University,Nantong 226001,China;?2.Laboratory of Image Engineering,Medical school of Nantong Univeristy,Nantong 226001,China;3.Key Laboratory of Neural Regeneration of Jiangsu Province,Nantong University,Nantong 226001,China)  
Objective With personal computer(PC) as the platform,to explore an computerized 3D reconstruction method based on rat's cervical cord serial sections. Methods Normal cervical cord segment of SD rat was embeded in paraffin block,four locating marks were made at four corners around the specimen,the specimen was sectioned serially with Leica paraffin microtome,after each three sections,the images of specimen and outer locating marks were taken with CANON digital camera and the serial sections were obtained.The obtained images were ordered,cut,turned into gray scale images and background uneven corrected.The edges of white matter,grey matter and locating marks in each image were extracted,the locating marks were used for automated registration of serial sections with 3D-DOCTOR software,the 3D surface reconstruction of cervical cord segment was observed,cut and measured freely in PC. Results The grey matter,white matter of rat cervical cord segment were reconstructed from serial transverse sections,the reconstructed segment was measured to obtain the surface area and volume data.Conclusion The paraffin embedding and "hard" locating marks techniques can be used to obtain the images of serial transverse sections of rat's cerival cord segment,to make 3D reconstruction which can be observed and measured freely.
【Fund】: 江苏省高校自然科学基金项目(05KJB180105);; 江苏省神经再生重点实验室开放课题(JSK-04003);; 南通市社会发展基金项目(S40052)资助
【CateGory Index】: TP391.41
Download(CAJ format) Download(PDF format)
CAJViewer7.0 supports all the CNKI file formats; AdobeReader only supports the PDF format.
©2006 Tsinghua Tongfang Knowledge Network Technology Co., Ltd.(Beijing)(TTKN) All rights reserved