PRODUCTION OF THE SPECIFIC ANTIBODY TO NEUROPEPTIDE Y
Su Huici, Huang Weiquan, Duan Xiaoqing, Sun Lan (Department of Histology & Embryology, Fourth Military Medical University)
Synthetic Neuropeptide Y(NPY) was coupled to bovine serum albumin (BSA) by glu(?)araldehyde. Antiserum was raised by immunising New Zealand rabbits with the NPY-BSA complex. The antiserum was tested with the immunohystochemistry ABC technique. It showed positive nerve fibres around the blood vessels in the rat gastrointestinal tract and the cerebral arteries. Positive nerve fibres and neuronal cell bodies were also found in the rat brain.The working dilution was 1:1, 000-1:2, 000.The pattern of the positive staining obtained with the antiserum was the same as that with a specific antiserum to NPY.The substitution and absorption tests were negative. The results suggeste that the raised antiserum is a specific antiserum to NPY with a high titre.