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《Chinese Journal of Anatomy》 1995-03
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CULTURE AND PURIFICATION OF SCHWANN CELLS FROM HUMAN FETAL SPINAL GANGLION

Yan Henglin, Shen Xinya, Liu Caidong, Chen Lilian, Wang Yang, Wang Zhimei (Department of Anatomy. Shanghai Medical University, Shanghai 200032)  
We have developed a simple and comparatively rapid method of collecting sufficient quantities of Schwann cells from human fetal spinal ganglion (SG) with a favorable purification ratio. Lumbar SG were cut into 0.5mm3 pieces, explanted on rat tail collagen coated coverslip and re-explanted 2 or 3 times on to new coverslip every- 2-3 days to get rid of fibroblasts contamination. The last re -explanted tissues were fed with DMEM supplemented with 20μg/ml bovine pituitary extract for 7-10 days. 5×106 Schwann cells were collected from one SG. Over 96% cells were typical bipoler and anti - S-100 protein positive. The Schwann cells obtained by this method were observed under both scanning and transmissing electron microscope in order to further display its typical morphological characteristics.
【Fund】: 国家自然科学基金资助项目
【CateGory Index】: R329
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