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《Chinese Journal of Anatomy》 2008-06
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Proliferation of neuronal precursor cells in serum-free culture

Zhou Junsong,Dong Yunhai,Song Xuehui,Xin Lian,Zhang Huanxiang(Medical College of Soochow University,Jiangsu Province Key Laboratory of Stem Cell Research,Suzhou 215123,China)  
Objective:To establish mixed primary culture system of cells from the cerebral cortex of newborn SD rats and study on the proliferation of neuronal precursor cells.Methods:Mixed primary cultures were prepared from the cerebral cortex of newborn SD rats; Prior to being cultured in serum-free medium,the mixed cells were maintained in serum medium for 6 days. Immunofluorescence staining was used to characterize the mixed cultures at different time points; BrdU was used to detect the proliferation of the neuronal precursor cells. Results:After being cultured for 6 days,glial cells were of the most abundant cell type in the primary mixed neuronal-glial cultures of newborn rat cortex and form a cell monolayer. After the serum medium was changed into the serum-free medium,neuronal precursor cells grew and proliferated on the top of the monolayer; The percentage of neurons and neuronal precursor increased significantly while that of neural stem cells and astrocytes decreased; The percentage of proliferative cells increased significantly in the first three days while it decreased in the following days. Conclusion:Mixed primary cultures in the serum-free medium can promote the proliferation of the neuronal precursor cells.
【Fund】: 国家自然科学基金(30671041);; 973项目(2005CB623902);; 江苏省高校自然科学重大基础研究项目(06KJA18025);; 江苏省高校自然科学基金一般项目(05KJB310115)
【CateGory Index】: R329
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